Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

Yes, our kits have been used successfully with robots in many countries.

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.