May I use heat-treated serum samples? ( 4 July 2013 )

For analysis by the complement fixation test, sera may require heat decomplementation (56°C for 30 minutes).  These heat-treated samples may also be tested by ELISA, although the decomplementation procedure may cause an increase in the background signal.

Can I use hemolysed serum/plasma samples? ( )

Yes, low to moderate hemolysis does not in general affect the ELISA performance.