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What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)? ( 4 July 2013 )

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they may be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet. 

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a joint, such as a cryotube). Otherwise, auto-lyophilisation may occur after 6-12 months at -20°C.

What is the purpose of the freeze-dried positive and negative sera (product codes beginning with “MRI”)? ( )

As it is often difficult for routine laboratories to obtain reference standards, and as international standards do not exist for all veterinary diseases, IDvet offers freeze-dried serum standards which may be used to check that the test’s analytical sensitivity does not vary between runs, operators and batches. Please contact IDvet for more information. Please refer to the List of positive and negative sera available from IDvet.pdf.

Are the IDvet ELISAs calibrated against international and national serum standards? ( )

Yes. IDvet tests meet international requirements for analytical sensitivity, as outlined in the table below:

Product nameProduct codeDirective or Standard
RUMINANTS
ID Screen® Brucellosis Serum Indirect Multi-speciesBRUS-MSReference serum OIEELISASPSS according to Commission Decision of 10 December 2008, 2008/984/CE, Annex C
ID Screen® Brucellosis Milk IndirectBRUMILK
Rose BengalRSA-RBReference sera OIEISS according to Commission Decision of 10 December 2008, 2008/984/CE, Annex C
Brucellosis Antigen for Complement Fixation TestAG-BRU
ID Screen® BLV CompetitionBLVCReference serum E05 according to  Commission Decision of 15 December 2009, 2009/976/EU, Chapter II Annex D
ID Screen® BLV IndirectBLVS
IDvet BLV AGIDBLVAGID
ID Screen IBR IndirectIBRSEU reference sera EU1, EU2 and EU3(1)
ID Screen® IBR gB CompetitionIBRGBC
ID Screen® IBR Milk IndirectIBRMILKFrench Reference Milk according to the OIE Terrestrial Manual 2010, Chapter 1.4.13
ID Screen® IBR gE CompetitionIBRGECEU reference sera EU1, EU2 and EU3(1)
SWINE
ID Screen® Aujeszky gB CompetitionAUJESZKYGBReference serum ADV 1 according to Commission Decision of 21 February 2008, 2008/184/EC, Annex III
ID Screen® Aujeszky gE CompetitionAUJESZKYGE
ID Screen® Classical Swine Fever E2 CompetitionCSFE2CPanel reference sera according to Commission Decision of 1 February 2002, 2002/106/EC, Chapter VII
ID Screen® Swine Vesicular Disease CompetitionSVDCReference serum RS 01-04-94 according to Commission Decision of 4 July 2000, 200/428/EC, Chapter X

(1) Perrin B., et al (1994). Selection of European Union standard reference sera for use in the serological diagnosis of infectious bovine rhinotracheitis. Rev. sci.tech. Off int. Epiz., 13(3), 947-960.