ID Screen® West Nile IgM Capture

IgM Antibody Capture ELISA (MAC) kit for the detection of anti-prE IgM antibodies in horse serum and plasma. The detection of IgM antibodies indicates recent infection and WNV circulation.

The monoclonal antibody used in the kit cross-reacts with other Japanese Encephalitis viruses. Please contact IDvet for more information.

 

  • Sensitive and specific detection of WNV IgM antibodies, indicators of recent infection 
  • Rapid and convenient: all reagents are supplied ready-to-use, samples are diluted directly in the plate (no pre-dilutions required), and all reagents may be transported at room temperature and stored at 4°C
  • Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera
Specifications
MethodIgM Antibody Capture ELISA
SpeciesEquine
SpecimensSerum and plasma
Coated antibodyAnti-horse IgM polyclonal antibody
AntigenReady-to-use WNV prE antigen
ConjugateAnti-WNV prE HRP conjugate (10X)
Protocol(1) Sample Incubation
(2) Three washes
(3) WNV ready-to-use antigen incubation
(4) Anti-WNV prE-HRP conjugate incubation
(5) Three washes
(6) Substrate Incubation: 15 min
Ordering information
Product codeWNIGM-1PWNIGM-4P
Kit format1 plate (bi-well format)4 plates (bi-well format)
Reactions48192
Plate format6 x 16-well strips6 x 16-well strips
References
  1. Zeller, H.G. and Schuffenecker, I. West Nile Virus: An overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis (2004) 23:147-156
  2. OIE Terrestrial Manual 2008 ; 2.1.20; West Nile Fever.
Associated products
West Nile IgM positive freeze-dried serum
Product codeMRI-WNIGM
Format0.5 ml vial (freeze-dried)
Description

Freeze-dried positive horse serum to be used as internal reference material for quality control.