IgM Antibody Capture ELISA (MAC) kit for the detection of anti-prE IgM antibodies in horse serum and plasma. The detection of IgM antibodies indicates recent infection and WNV circulation.

 

• Sensitive and specific detection of WNV IgM antibodies, indicators of recent infection 
• Rapid and convenient: all reagents are supplied ready-to-use, samples are diluted directly in the plate (no pre-dilutions required), and all reagents may be transported at room temperature and stored at 4°C
• Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera

Specifications
Method	IgM Antibody Capture ELISA
Species	Equine
Specimens	Serum and plasma
Coated antibody	Anti-horse IgM polyclonal antibody
Antigen	Ready-to-use WNV prE antigen
Conjugate	Anti-WNV prE HRP conjugate (10X)
Protocol	(1) Sample Incubation (2) Three washes (3) WNV ready-to-use antigen incubation (4) Anti-WNV prE-HRP conjugate incubation (5) Three washes (6) Substrate Incubation: 15 min

Ordering information
Product code	WNIGM-1P	WNIGM-4P
Kit format	1 plate (bi-well format)	4 plates (bi-well format)
Reactions	48	192
Plate format	6 x 16-well strips	6 x 16-well strips

References
Zeller, H.G. and Schuffenecker, I. West Nile Virus: An overview of its spread in Europe and the Mediterranean basin in contrast to its spread in the Americas. Eur J Clin Microbiol Infect Dis (2004) 23:147-156 OIE Terrestrial Manual 2008 ; 2.1.20; West Nile Fever.