Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species.

The monoclonal antibody used in the kit cross-reacts with other Japanese Encephalitis viruses and the tick-borne encephalitis virus.

 

• Highly sensitive and specific detection of WNV antibodies
• Applicable to multiple species, including horses, birds and others. For research use only on human samples: see References (*).
• Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours

Specifications
Method	Competitive ELISA
Species	Multiple species, including birds, horses and others. For research use only on human samples: see References (*).
Specimens	Serum or plasma
Coated antigen	pr-E antigen
Conjugate	Anti-pr-E-HRP conjugate (concentrated 10X)

Ordering information
Product code	WNC-1P	WNC-2P
Kit format	1 plate	2 plates
Reactions	96	192
Plate format	12 x 8-well strips	12 x 8-well strips

References

OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever. Horses Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France. Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007. Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101. Birds Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009. Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011. Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012), http://dx.doi.org/10.1016/j.vetmic.2012.07.041. Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journal of Animal and Veterinary Advances 11 (13):221-2214,2012 Other species Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China. Arch Virol (2011) 156:893-895. (*) Niczyporuk J.,1 Samorek-Salamonowicz E. et al. ( 2015) Occurrence of West Nile Virus Antibodies in Wild Birds, Horses, and Humans in Poland. BioMed Research International vol 2015, Article ID 234181 More external references available upon request.