ID Screen® West Nile Competition Multi-species

Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species. The monoclonal antibody used in the kit cross-reacts with other Japanese Encephalitis viruses and the tick-borne encephalitis virus.

Please contact IDvet for more information.

 

  • Highly sensitive and specific detection of WNV antibodies
  • Applicable to multiple species, including horses, birds and others.
  • Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours
Specifications
Method Competitive ELISA
Species Multiple species, including birds, horses and others
Specimens Serum or plasma
Coated antigen pr-E antigen
Conjugate Anti-pr-E-HRP conjugate (concentrated 10X)
Ordering information
Product code WNC-1P WNC-2P
Kit format 1 plate 2 plates
Reactions 96 192
Plate format 12 x 8-well strips 12 x 8-well strips
References
  1. OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever.
Horses
  1. Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France.
  2. Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007.
  3. Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101.
Birds
  1. Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009.
  2. Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011.
  3. Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012), http://dx.doi.org/10.1016/j.vetmic.2012.07.041.
  4. Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journal of Animal and Veterinary Advances 11 (13):221-2214,2012
Other species
  1. Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China. Arch Virol (2011) 156:893-895.
Associated reference sera
West Nile IgG positive freeze-dried serum
Product code MRI-WN
Format 1 ml vial (freeze-dried)
Description

Freeze-dried positive horse serum to be used as internal reference material for quality control.
SPF negative freeze-dried serum
Product code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description

Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

May be used for ELISA and RSA testing.