Competitive ELISA kit for the detection of anti-pr-E antibodies in multiple species.
The monoclonal antibody used in the kit cross-reacts with other Japanese Encephalitis viruses and the tick-borne encephalitis virus.
- Highly sensitive and specific detection of WNV antibodies
- Applicable to multiple species, including horses, birds and others. For research use only on human samples: see References (*).
- Rapid and convenient protocol, with ready-to-use reagents and results in just over 2 hours
| Specifications |
|---|
| Method | Competitive ELISA |
| Species | Multiple species, including birds, horses and others. For research use only on human samples: see References (*). |
| Specimens | Serum or plasma |
| Coated antigen | pr-E antigen |
| Conjugate | Anti-pr-E-HRP conjugate (concentrated 10X) |
| Ordering information |
|---|
| Product code | WNC-1P | WNC-2P |
| Kit format | 1 plate | 2 plates |
| Reactions | 96 | 192 |
| Plate format | 12 x 8-well strips | 12 x 8-well strips |
| References |
|---|
- OIE Terrestrial Manual 2009; 2.1.20; West Nile Fever.
Horses- Van Maanen, Kees et al. Surveillance of West Nile Virus incursions in the Netherlands: Validation of antibody detecting ELISAs in horses. Oral presentation at the 2010 Epizone meeting in Saint Malo, France.
- Wernery, U. et al. West Nile Fever in the United Arab Emirates. Wildlife in the Middle East. Vol. 2, Issue 3, December 2007.
- Ziegler et al. Monitoring of West Nile Virus Infections in Germany. Zoonoses and Public Health 59 (Suppl. 2) (2012) 95-101.
Birds- Diaz-Sanchez, S. et al. Use of a multispecies competitive ELISA for investigation of exposure to West Nile virus in multiple species of captive and wild birds. Presented at the WAVLD meeting, Madrid 2009.
- Garcia-Bocanegra et al. Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain. Vector-Borne and Zoonotic Diseases. Volume 11, Number 0, 2011.
- Ziegler et al. Pathogenesis of West Nile virus lineage 1 and 2 in experimentally infected large falcons. Vet. Microbiol. (2012), http://dx.doi.org/10.1016/j.vetmic.2012.07.041.
- Yipici et al. Serologic Investigation for West Nile Virus Infection in Commerical Domestic Chickens (Gallus gallus domesticus). Journal of Animal and Veterinary Advances 11 (13):221-2214,2012
Other species- Lan et. al. 2011. Serological evidence of West Nile virus in dogs and cats in China. Arch Virol (2011) 156:893-895.
- (*) Niczyporuk J.,1 Samorek-Salamonowicz E. et al. ( 2015) Occurrence of West Nile Virus Antibodies in Wild Birds, Horses, and Humans in Poland. BioMed Research International vol 2015, Article ID 234181
More external references available upon request. |
| West Nile IgG positive freeze-dried serum |
| Product code | MRI-WN |
| Format | 1 ml vial (freeze-dried) |
| Description | Freeze-dried positive horse serum to be used as internal reference material for quality control. |
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