ID Screen® Schmallenberg virus Indirect Multi-species

Indirect ELISA kit for the detection of anti-Schmallenberg virus (SBV) antibodies in ruminant serum and plasma from multiple species, including cattle, sheep and goats.

Please contact IDvet for use on other species.

 

  • The first commercial ELISA for SBV serology
  • High sensitivity and specificity in external validation studies
  • Approved by the ANSES (France) and FLI (Germany) laboratories
  • Automatable and therefore suited to high throughput testing
  • Screening and confirmation formats available
Specifications
Method Indirect ELISA
Species Ruminants and other species
Specimens Serum and plasma
Coated antigen Recombinant SBV antigen
Conjugate Anti-multi-species IgG-HRP conjugate (concentrated 10X)
Ordering information
Screening format
Product code SBVS-2P SBVS-5P
Kit format 2 plates 5 plates
Reactions 192 480
Plate format 12 x 8-well strips 12 x 8-well strips
Confirmation format *
Product code SBVB-4P SBVB-10P
Kit format 4 plates (bi-well) 10 plates (bi-well)
Reactions 192 480
Plate format 6 x 16 well strips 6 x 16 well strips
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
References
  1. Jack C., Anstaett O, Adams J, Noad R, Browlie J., Mertens P. Evidence of seroconversion to SBV in camelids. Veterinary Record 2012 170:603 doi: 10.1136/vr.e3939
  2. Kaba et al. Schmallenberg Virus Antibodies Detected in Poland. Transboundary and Emerging Diseases. Rapid Communication. 2012.
  3. Linden A, Desmecht D, Volpe R, Wirtgen M, Pirson J, Paternostre J, et al. Epizootic Spreads of Schmallenberg virus among wild cervids, Belgium, fall 2011. Emerg Infect Dis (Internet). 2012 Dec.
  4. Bréard E, Lara E, Comtet L, Viarouge C, Doceul V, et al. (2013) Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies. PLoS ONE 8(1): e53446. doi:10.1371/journal.pone.0053446
Associated reference sera
Schmallenberg virus positive freeze-dried serum
Product code MRI-SBV
Format 1 ml vial (freeze-dried)
Description

Freeze-dried positive bovine serum to be used as internal reference material for quality control.
Negative bovine freeze-dried serum
Product code MRINEG-BOV
Format 1 ml vial (freeze-dried)
Description

Negative freeze-dried bovine serum to be used as internal reference material for quality control.