Indirect ELISA kit for the detection of anti-Schmallenberg virus (SBV) antibodies in ruminant serum and plasma from multiple species, including cattle, sheep and goats.
Please contact IDvet for use on other species.
- The first commercial ELISA for SBV serology
- High sensitivity and specificity in external validation studies
- Approved by the ANSES (France) and FLI (Germany) laboratories
- Automatable and therefore suited to high throughput testing
- Screening and confirmation formats available
| Specifications |
|---|
| Method | Indirect ELISA |
| Species | Ruminants and other species |
| Specimens | Serum and plasma |
| Coated antigen | Recombinant SBV antigen |
| Conjugate | Anti-multi-species IgG-HRP conjugate (concentrated 10X) |
| Screening format |
| Product code | SBVS-2P | SBVS-5P |
| Kit format | 2 plates | 5 plates |
| Reactions | 192 | 480 |
| Plate format | 12 x 8-well strips | 12 x 8-well strips |
| Confirmation format * |
| Product code | SBVB-5P | SBVB-10P |
| Kit format | 5 plates (bi-well) | 10 plates (bi-well) |
| Reactions | 240 | 480 |
| Plate format | 6 x 16 well strips | 6 x 16 well strips |
* Each sample is tested in duplicate in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD value (even well OD – odd well OD).
| References |
|---|
- Jack C., Anstaett O, Adams J, Noad R, Browlie J., Mertens P. Evidence of seroconversion to SBV in camelids. Veterinary Record 2012 170:603 doi: 10.1136/vr.e3939
- Kaba et al. Schmallenberg Virus Antibodies Detected in Poland. Transboundary and Emerging Diseases. Rapid Communication. 2012.
- Linden A, Desmecht D, Volpe R, Wirtgen M, Pirson J, Paternostre J, et al. Epizootic Spreads of Schmallenberg virus among wild cervids, Belgium, fall 2011. Emerg Infect Dis (Internet). 2012 Dec.
- Bréard E, Lara E, Comtet L, Viarouge C, Doceul V, et al. (2013) Validation of a Commercially Available Indirect Elisa Using a Nucleocapside Recombinant Protein for Detection of Schmallenberg Virus Antibodies. PLoS ONE 8(1): e53446. doi:10.1371/journal.pone.0053446
|
| Associated reference sera |
|---|
| Schmallenberg virus positive freeze-dried serum |
| Product code | MRI-SBV |
| Format | 1 ml vial (freeze-dried) |
| Description | Freeze-dried positive bovine serum to be used as internal reference material for quality control. |
| Negative bovine freeze-dried serum |
| Product code | MRINEG-BOV |
| Format | 1 ml vial (freeze-dried) |
| Description | Negative freeze-dried bovine serum to be used as internal reference material for quality control. |
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