IDvet offers a complete solution for Bovine TB diagnosis via the IGRA method, including avian and bovine PPD tuberculin antigens and the ID Screen® Ruminant IFN-g sandwich ELISA. Download our product brochure.
Sandwich ELISA for the detection of ovine, bovine and caprine interferon gamma (IFN-g) in activated plasma or culture supernatant.
- Standardized results thanks to a freeze-dried positive reference control used to calculate S/P ratios for each sample
- Flexible: the same kit may be used with both native antigens (standard protocol) and recombinant / peptide antigens (alternative protocol)
- Practical: the standard protocol uses only 10μl per sample, meaning that less sample volume is required for cell activation
| Specifications |
|---|
| Method | Sandwich ELISA |
| Species | Bovine, ovine and caprine |
| Specimens | Activated plasma or culture supernatant |
| Coated antibody | Anti-ruminant IFN-g monoclonal antibody |
| Conjugate | Anti-ruminant IFN-g HRP Mab conjugate |
| Ordering information |
|---|
| Product code | IFNG-2P | IFNG-4P | IFNG-10P |
| Kit format | 2 plates | 4 plates | 10 plates |
| Reactions | 192 | 384 | 960 |
| Plate format | 12 x 8-well strips | 12 x 8-well strips | 12 x 8-well strips |
| References |
|---|
- Comtet, L., Pourquier, P. (IDvet). ID Screen® Interferon gamma ELISA: improvement of analytical sensitivity and introduction of a standard reference control to improve result interpretation. Poster presented at the 2012 International Colloquium on Paratuberculosis, Sydney, Australia.
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| Associated reference sera |
|---|
| Interferon gamma positive freeze-dried bovine activated plasma |
| Product code | MRI-IFNG-B |
| Format | 0.5 ml vial (freeze-dried) |
| Description | Positive freeze-dried bovine activated plasma to be used as internal reference material for quality control. |
| Interferon gamma positive freeze-dried ovine activated plasma |
| Product code | MRI-IFNG-O |
| Format | 0.5 ml vial (freeze-dried) |
| Description | Positive freeze-dried ovine activated plasma to be used as internal reference material for quality control. |
| Interferon gamma positive freeze-dried caprine activated plasma |
| Product code | MRI-IFNG-C |
| Format | 0.5 ml vial (freeze-dried) |
| Description | Positive freeze-dried caprine activated plasma to be used as internal reference material for quality control. |
| Interferon gamma positive freeze-dried buffalo activated plasma |
| Product code | MRI-IFNG-BF |
| Format | 0.5 ml vial (freeze-dried) |
| Description | Positive freeze-dried buffalo activated plasma to be used as internal reference material for quality control. |
| Interferon gamma negative freeze-dried plasma |
| Product code | MRINEG-IFNG |
| Format | 1 ml vial (freeze-dried) |
| Description | Freeze-dried bovine plasma with no interferon gamma, to be used as internal reference material for quality control. |
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