Veterinary Diagnostics

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Peste des Petits Ruminants Virus (PPRV)

ID Screen® PPR Antigen Capture

ELISA

Sandwich ELISA for the detection of Peste des Petits Ruminants virus in live animals or for post-mortem diagnosis

This test does not contain any virus particles.

Advantages

Specifications

Format

References

Downloads

  • Equivalent performance to RT-PCR
  • Improved sensitivity for samples with weak or strong PPRV titres
  • Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
  • Convenient and easy-to-use: microplates are supplied coated in strips of 8 wells
  • Straightforward and rapid protocol, with results in 90 minutes
  • Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera

Method :

Sandwich ELISA

Species :

Ovine and caprine

Specimens :

Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC

Coated antigen :

Anti-PPRV NP antibody

Conjugate :

Anti-PPRV-NP Mab-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

PPRAG-1P 1 plate 96 12 x 8-well strips
PPRAG-2P 2 plates 192 12 x 8-well strips
  1. Jemberu W.T. et al. (2022). Economic impact of a peste des petits ruminants outbreak and vaccination cost in northwest Ethiopia. Transboundary and Emerging Diseases.
  2. Ahmed S. et al. (2021). Isolation and identification of peste des petits ruminants virus from goats in Egyptian governorates. Veterinary World, 14(4), 926.
  3. Haroun M. et al. (2021). Peste Des Petits Ruminants: A First Retrospective Investigation Among Susceptible Animal Species in Qatar. DOI: https://doi.org/10.21203/rs.3.rs-371540/v1.
  4. Saeed F. A. et al. (2021). Epidemiology and molecular characterization of re-emerged virulent strains of Peste des Petits Ruminants virus among sheep in Kassala State, Eastern Sudan. Irish Veterinary Journal, 74(1), 1-9.
  5. Halecker S. et al. (2020). Comparative evaluation of different antigen detection methods for the detection of peste des petits ruminants virus. Transboundary and Emerging Diseases, 67(6), 2881-2891.
  6. Manzoor S. et al. (2020). Genetic characterization of peste des petits ruminants virus (Pakistani isolates) and comparative appraisal of diagnostic assays. Transboundary and Emerging Diseases, 67(5), 2126-2132.
  7. Asil R. M. et al. (2019). First detection and genetic characterization of peste des petits ruminants virus from dorcas gazelles “Gazella dorcas” in the Sudan, 2016-2017. Archives of Virology, 164(10), 2537-2543.
  8. Bataille A. et al. (2019). Optimization and evaluation of a non-invasive tool for PPR surveillance and control. Scientific Reports 9: 4742.
  9. Halecker S. et al. (2019). Studies on the evaluation of a molecular PEN-SIDE TEST for PPRV. 13th EPIZONE Annual Meeting, 26-28 August 2019, Berlin, Germany.
  10. Donduashvili M. et al. (2018). Identification of Peste des Petits Ruminants Virus, Georgia, 2016. Emerging Infectious Diseases, 24(8):1576-1578.
  11. Bodisaikhan K. (2017) PESTE DES PETITS RUMINANTS (PPR) IN SAIGA ANTELOPE IN MONGOLIA. OIE communication Bali, Indonesia. 2017.07.04-06.
  12. Mahmoud, A.Z.E. et al. (2017). Outbreaks of PPR-FMD among sheep and goats in Hail, Saudi Arabia. Veterinary Sciences: Research and Reviews, 3(2): 38-44.
  13. Karim A. et al. (2016). Detection of Peste des petits ruminants virus and goatpox virus from an outbreak in goats with high mortality in Meghalaya state, India. Veterinary World, 9(9): 1025-1027.
  14. Zakian A. et al. (2016). The first report of peste des petits ruminants (PPR) in camels (Camelus dromedarius) in Iran. Trop Anim Health Prod.1007/s11250-016-1078-6.
  15. Rojas J. M. et al. (2014). Vaccination with Recombinant Adenoviruses Expressing the Pest des Petits Ruminants Virus F or H Proteins Overcomes Viral Immunosuppression and Induces Protective Immunity against PPRV Challenge in Sheep. Plos One, Vol. 9, Issue 7, e101226.
  16. Comtet L. et al. (2013). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the 7th Epizone Annual Meeting 2013. Brussels, Belgium.Satrovic L. et al. (2022).

Associated products

Internal reference material
ELISA