Sandwich ELISA for the detection of Peste des Petits Ruminants virus in live animals or for post-mortem diagnosis.

Based on reagents developed at a FAO/OIE PPRV reference laboratory (CIRAD, Montpellier, France). IDvet contributed to the improvement of this test by optimizing test performance and component stability.

 

• This test does not contain any virus particles
• Equivalent performance to RT-PCR (7)
• Improved sensitivity for samples with weak or strong PPRV titres
• Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
• Convenient and easy-to-use: microplates are supplied coated in strips of 8 wells
• Straightforward and rapid protocol, with results in 90 minutes
• Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera

Specifications
Method	Sandwich ELISA
Species	Ovine and caprine
Specimens	Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
Coated antibody	Anti-PPRV NP antibody
Conjugate	Anti-PPRV-NP Mab-HRP conjugate (concentrated 10X)

Ordering information
Product code	PPRAG-1P	PPRAG-2P
Kit format	1 plate	2 plates
Reactions	96	192
Plate format	12 x 8-well strips	12 x 8-well strips

References

O. Kwiatek, Y.H. Ali, I.K. Saeed, A.I. Khalafalla, O.I. Mohamed, A.A. Obeida, M.B. Abdelrahman, H.M. Osman, K.M. Taha, Z. Abbas, M. El Harrak, Y. Lhor, A. Diallo, R. Lancelot, E. Albina, G. Libeau. Asian lineage of peste des petits ruminants virus, Africa. Emerg. Infect. Dis., 17 (2011), pp. 1223–1231 Recognizing peste des petits ruminants - A field manual, FAO, section: diagnosis of PPR. URL link: http://www.fao.org/docrep/003/x1703e/x1703e00.htm#Diagnosis of PPR Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet.Rec. 19, 300–304 Abubakar, M., et al. Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants. Bulgarian Journal of Veterinary Medicine (2011), 14, No 2, 103-108 Couacy-Hymann, E., et al. The early detection of peste-des-petits-ruminants (PPR) virus antigens and nucleic acid from experimentally infected goats using RT- PCR and immunocapture ELISA techniques. Research in Veterinary Science, Volume 87, Issue 2, October 2009, 332335 Muhammad M., et al. (2012). Serodiagnosis of Peste des Petits Ruminants Virus, Serological Diagnosis of Certain Human, Animal and Plant Diseases, Dr. Moslih Al-Moslih (Ed.), ISBN: 978-953-51-0370-7, InTech, Available from: http://www.intechopen.com/books/serologicaldiagnosis-of-certain-human-animal-and-plant- diseases/serodiagnosis-of-peste-des-petits-ruminants-virus Comtet, L., Pourquier, P. (IDvet, France), Kwiatek, O., Libeau, G. (CIRAD, France). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the Epizone 7th Annual Meeting, Brussels, Belgium, 2013.