ID Screen® PPR Antigen Capture

Sandwich ELISA for the detection of Peste des Petits Ruminants virus in live animals or for post-mortem diagnosis.

Based on reagents developed at a FAO/OIE PPRV reference laboratory (CIRAD, Montpellier, France). IDvet contributed to the improvement of this test by optimizing test performance and component stability.

 

  • This test does not contain any virus particles
  • Equivalent performance to RT-PCR (7)
  • Improved sensitivity for samples with weak or strong PPRV titres
  • Flexible test for use with a wide variety of matrices, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
  • Convenient and easy-to-use: microplates are supplied coated in strips of 8 wells
  • Straightforward and rapid protocol, with results in 90 minutes
  • Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes color upon addition of the sera
Specifications
Method Sandwich ELISA
Species Ovine and caprine
Specimens Variety of sample types, including oral, nasal or rectal swabs as well as tears, tissue samples, gum debris or PBMC
Coated antibody Anti-PPRV NP antibody
Conjugate Anti-PPRV-NP Mab-HRP conjugate (concentrated 10X)
Ordering information
Product code PPRAG-1P PPRAG-2P
Kit format 1 plate 2 plates
Reactions 96 192
Plate format 12 x 8-well strips 12 x 8-well strips
References
  1. O. Kwiatek, Y.H. Ali, I.K. Saeed, A.I. Khalafalla, O.I. Mohamed, A.A. Obeida, M.B. Abdelrahman, H.M. Osman, K.M. Taha, Z. Abbas, M. El Harrak, Y. Lhor, A. Diallo, R. Lancelot, E. Albina, G. Libeau. Asian lineage of peste des petits ruminants virus, Africa. Emerg. Infect. Dis., 17 (2011), pp. 1223–1231
  2. Recognizing peste des petits ruminants - A field manual, FAO, section: diagnosis of PPR. URL link: http://www.fao.org/docrep/003/x1703e/x1703e00.htm#Diagnosis of PPR
  3. Libeau, G., Diallo, A., Colas, F., Guerre, L., 1994. Rapid differential diagnosis of rinderpest and peste des petits ruminants using an immunocapture ELISA. Vet.Rec. 19, 300–304
  4. Abubakar, M., et al. Comparison of antigen detection methods of peste des petits ruminants virus in clinical samples of small ruminants. Bulgarian Journal of Veterinary Medicine (2011), 14, No 2, 103-108
  5. Couacy-Hymann, E., et al. The early detection of peste-des-petits-ruminants (PPR) virus antigens and nucleic acid from experimentally infected goats using RT- PCR and immunocapture ELISA techniques. Research in Veterinary Science, Volume 87, Issue 2, October 2009, 332335
  6. Muhammad M., et al. (2012). Serodiagnosis of Peste des Petits Ruminants Virus, Serological Diagnosis of Certain Human, Animal and Plant Diseases, Dr. Moslih Al-Moslih (Ed.), ISBN: 978-953-51-0370-7, InTech, Available from: http://www.intechopen.com/books/serologicaldiagnosis-of-certain-human-animal-and-plant- diseases/serodiagnosis-of-peste-des-petits-ruminants-virus
  7. Comtet, L., Pourquier, P. (IDvet, France), Kwiatek, O., Libeau, G. (CIRAD, France). Validation of the ID Screen® Peste des Petits Ruminants Antigen Capture ELISA. Poster presented at the Epizone 7th Annual Meeting, Brussels, Belgium, 2013.