Indirect absorbed (3) screening ELISA for the detection of anti-Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk.
- Ideal for herd certification and disease eradication programs thanks to high sensitivity, specificity and clear separation of positive and negative results
- Automatable and rapid : results in 90 minutes.
- Screening and confirmation formats available
Specifications |
Method |
Indirect absorbed ELISA |
Species |
Ovine, caprine, bovine (cattle or buffalo) |
Specimens |
Serum, plasma or bovine milk |
Coated antigen |
Purified MAP extract |
Conjugate |
Anti-ruminant-HRP conjugate (concentrated 10X) |
Incubations |
Short or overnight |
Screening format |
Product code |
PARAS-5P |
PARAS-10P |
Kit format |
5 plates |
10 plates |
Reactions |
480 |
960 |
Plate format |
12 x 8-well strips |
12 x 8-well strips |
Confirmation format * |
Product code |
PARAB-5P |
Kit format |
5 plates bi-well |
Reactions |
240 |
Plate format |
6 x 16-well strips |
* Each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD (even well OD – odd well OD).
References |
- Köhler, Heike et al. Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210 (2008).
- Fry, M., Kruze, J., Collins, M., Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332 (2008).
- OIE recommendations: Manual of Recommended Diagnostic Techniques and Requirements volume III -Paratuberculosis 5B/009.
|
Freeze-dried Paratuberculosis positive milk |
Product code |
MRI-PARAMILK |
Format |
2 ml vial (freeze-dried) |
Description |
Pool of freeze-dried bovine milks containing anti-Mycobacterium avium subsp. paratuberculosis (MAP) specific antibodies.
|
Paratuberculosis positive freeze-dried serum |
Product code |
MRI-PARA |
Format |
1 ml vial (freeze-dried) |
Description |
Freeze-dried positive bovine serum to be used as internal reference material for quality control.
|
Paratuberculosis negative freeze-dried serum |
Product code |
MRINEG-PARA |
Format |
2 ml vial (freeze-dried) |
Description |
Freeze-dried negative bovine serum to be used as internal reference material for quality control.
|