ID Screen® Paratuberculosis Indirect

Indirect absorbed (3) screening ELISA for the detection of anti-Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk.

  • Ideal for herd certification and disease eradication programs thanks to high sensitivity, specificity and clear separation of positive and negative results
  • Automatable and rapid : results in 90 minutes.
  • Screening and confirmation formats available
Specifications
MethodIndirect absorbed ELISA
SpeciesOvine, caprine, bovine (cattle or buffalo)
SpecimensSerum, plasma or bovine milk
Coated antigenPurified MAP extract
ConjugateAnti-ruminant-HRP conjugate (concentrated 10X)
IncubationsShort or overnight
Ordering information
Screening format
Product codePARAS-5PPARAS-10P
Kit format5 plates10 plates
Reactions480960
Plate format12 x 8-well strips12 x 8-well strips
Confirmation format *
Product codePARAB-5P
Kit format5 plates bi-well
Reactions240
Plate format6 x 16-well strips
* Each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD (even well OD – odd well OD).
References
  1. Köhler, Heike et al. Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210 (2008).
  2. Fry, M., Kruze, J., Collins, M., Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332 (2008).
  3. OIE recommendations: Manual of Recommended Diagnostic Techniques and Requirements volume III -Paratuberculosis 5B/009.
Associated reference sera
Freeze-dried Paratuberculosis positive milk
Product codeMRI-PARAMILK
Format2 ml vial (freeze-dried)
Description

Pool of freeze-dried bovine milks containing anti-Mycobacterium avium subsp. paratuberculosis (MAP) specific antibodies.

Paratuberculosis positive freeze-dried serum
Product codeMRI-PARA
Format1 ml vial (freeze-dried)
Description

Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Paratuberculosis negative freeze-dried serum
Product codeMRINEG-PARA
Format2 ml vial (freeze-dried)
Description

Freeze-dried negative bovine serum to be used as internal reference material for quality control.