Indirect absorbed (3) screening ELISA for the detection of anti-Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk.
- Ideal for herd certification and disease eradication programs thanks to high sensitivity, specificity and clear separation of positive and negative results
- Automatable and rapid : results in 90 minutes.
- Screening and confirmation formats available
| Specifications |
|---|
| Method | Indirect absorbed ELISA |
| Species | Ovine, caprine, bovine (cattle or buffalo) |
| Specimens | Serum, plasma or bovine milk |
| Coated antigen | Purified MAP extract |
| Conjugate | Anti-ruminant-HRP conjugate (concentrated 10X) |
| Incubations | Short or overnight |
| Screening format |
| Product code | PARAS-5P | PARAS-10P |
| Kit format | 5 plates | 10 plates |
| Reactions | 480 | 960 |
| Plate format | 12 x 8-well strips | 12 x 8-well strips |
| Confirmation format * |
| Product code | PARAB-5P |
| Kit format | 5 plates bi-well |
| Reactions | 240 |
| Plate format | 6 x 16-well strips |
* Each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD (even well OD – odd well OD).
| References |
|---|
- Köhler, Heike et al. Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210 (2008).
- Fry, M., Kruze, J., Collins, M., Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332 (2008).
- OIE recommendations: Manual of Recommended Diagnostic Techniques and Requirements volume III -Paratuberculosis 5B/009.
|
| Freeze-dried Paratuberculosis positive milk |
| Product code | MRI-PARAMILK |
| Format | 2 ml vial (freeze-dried) |
| Description | Pool of freeze-dried bovine milks containing anti-Mycobacterium avium subsp. paratuberculosis (MAP) specific antibodies. |
| Paratuberculosis positive freeze-dried serum |
| Product code | MRI-PARA |
| Format | 1 ml vial (freeze-dried) |
| Description | Freeze-dried positive bovine serum to be used as internal reference material for quality control. |
| Paratuberculosis negative freeze-dried serum |
| Product code | MRINEG-PARA |
| Format | 2 ml vial (freeze-dried) |
| Description | Freeze-dried negative bovine serum to be used as internal reference material for quality control. |
