ID Screen® Paratuberculosis Indirect

Indirect absorbed (3) screening ELISA for the detection of anti-Mycobacterium avium subsp. paratuberculosis (Map) antibodies in ruminant serum, plasma and milk.

  • Ideal for herd certification and disease eradication programs thanks to high sensitivity, specificity and clear separation of positive and negative results
  • Automatable and rapid : results in 90 minutes.
  • Screening and confirmation formats available
Specifications
Method Indirect absorbed ELISA
Species Ovine, caprine, bovine (cattle or buffalo)
Specimens Serum, plasma or bovine milk
Coated antigen Purified MAP extract
Conjugate Anti-ruminant-HRP conjugate (concentrated 10X)
Incubations Short or overnight
Ordering information
Screening format
Product code PARAS-5P PARAS-10P
Kit format 5 plates 10 plates
Reactions 480 960
Plate format 12 x 8-well strips 12 x 8-well strips
Confirmation format *
Product code PARAB-5P
Kit format 5 plates bi-well
Reactions 240
Plate format 6 x 16-well strips
* Each sample is tested in a coated well (even columns) and in an adjacent non-coated well (odd columns). Results are calculated with respect to the corrected sample OD (even well OD – odd well OD).
References
  1. Köhler, Heike et al. Evaluation of five ELISA test kits for the measurement of antibodies against Mycobacterium avium subspecies paratuberculosis in bovine serum. Berl. Münch. Tierärztl. Wochenschr. 121, Heft 5/6, 203-210 (2008).
  2. Fry, M., Kruze, J., Collins, M., Evaluation of four commercial enzyme-linked immunosorbent assays for the diagnosis of bovine paratuberculosis in Chilean dairy herds. J Vet Diagn Invest 20:329-332 (2008).
  3. OIE recommendations: Manual of Recommended Diagnostic Techniques and Requirements volume III -Paratuberculosis 5B/009.
Associated reference sera
Freeze-dried Paratuberculosis positive milk
Product code MRI-PARAMILK
Format 2 ml vial (freeze-dried)
Description

Pool of freeze-dried bovine milks containing anti-Mycobacterium avium subsp. paratuberculosis (MAP) specific antibodies.

Paratuberculosis positive freeze-dried serum
Product code MRI-PARA
Format 1 ml vial (freeze-dried)
Description

Freeze-dried positive bovine serum to be used as internal reference material for quality control.

Paratuberculosis negative freeze-dried serum
Product code MRINEG-PARA
Format 2 ml vial (freeze-dried)
Description

Freeze-dried negative bovine serum to be used as internal reference material for quality control.