Indirect ELISA kit for the detection of antibodies against Maedi Visna (MVV)/CAEV viruses in sheep and goats serum, plasma or milk samples.
The use of a mix of peptide antigens results in superior test performance (sensitivity, specificity, and differentiation between positive and negative results).
- Reliable and accurate : clearly separates positive and negative results, with high sensitivity – detection of all genotypes (including A; B and E) – and high specificity
- No non-specific reactions due to BTV-vaccination, thanks to the use of peptide antigens. Other ELISAs based on recombinant or purified viral antigens have given nonspecific reactions in BTV-vaccinated populations (1)
- Easy-to-use, with results in 90 minutes
|Species||Sheep and goats|
|Specimens||Serum, plasma, milk|
|Coated antigen||Panel of peptides from the MVV/CAEV TM, gp135 and p25 proteins|
|Conjugate||Anti-ruminant IgG-HRP conjugate (concentrated 10X)|
|Kit format||5 plates|
|Plate format||12 x 8-well strips|
- Comtet, L. (IDvet), Feliziani, F. (CEREL – IZS Umbria e Marche, Italy), Lesceu, S. (IDvet). Validation of the ID Screen® Maedi Visna Indirect ELISA: specificity on BTV-8 vaccinated sheep and detection of seroconversion. Poster presented at the 2010 EAVLD meeting, Lelystad, Holland.
|Associated reference sera|
|Freeze-dried MVV / CAEV reference milk|
Freeze-dried pool of ovine milks containing anti-MVV/CAEV specific antibodies. Contains bronopol (0.2%).
|MVV / CAEV positive freeze-dried serum|
|Product code||MRI-VISNA / CAEV|
|Format||0.5 ml vial (freeze-dried)|
Freeze-dried positive ovine and caprine serum pool to be used as internal reference material for quality control.