Veterinary Diagnostics

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Maedi-visna Virus (MVV) / Caprine Arthritis Encephalitis Virus (CAEV)

ID Screen® MVV / CAEV Indirect

ELISA

Indirect ELISA kit for the detection of antibodies against Maedi Visna (MVV)/CAEV viruses in sheep and goats serum, plasma or milk samples

The use of a mix of peptide antigens results in superior test performance (sensitivity, specificity, and differentiation between positive and negative results).

Advantages

Specifications

Format

References

Downloads

  • Reliable and accurate : clearly separates positive and negative results, with high sensitivity – detection of all genotypes (including A; B and E) – and high specificity
  • No non-specific reactions due to BTV-vaccination, thanks to the use of peptide antigens. Other ELISAs based on recombinant or purified viral antigens have given nonspecific reactions in BTV-vaccinated populations (1)
  • Easy-to-use, with results in 90 minutes

Method :

Indirect ELISA

Species :

Sheep and goats

Specimens :

Serum, plasma, milk

Coated antigen :

Panel of peptides from the MVV/CAEV TM, gp135 and p25 proteins

Conjugate :

Anti-ruminant IgG-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

VISNAS-5P 5 plates 480 12 x 8-well strips

GOATS

SERUM

  1. Kaba J. et al. (2022). Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams. Journal of Veterinary Research, 66(4), 511-521.
  2. Nardelli S. et al. (2020). Eradication of caprine arthritis encephalitis virus in the goat population of South Tyrol, Italy: Analysis of the tailing phenomenon during the 2016–2017 campaign. Journal of veterinary diagnostic investigation, 32(4), 589-593.
  3. Czopowicz M. et al. (2017). Fall in antibody titer to small ruminant lentivirus in the periparturient period in goats. Small Ruminant Research, 147, 37-40.
  4. Gumusova S. et al. (2016). Caprine Arthritis Encephalitis and Bluetongue Virus Infections in Maltese, Saanen and Hair Goat Breeds. Pakistan Journal of Zoology, 48(5).
  5. Nowicka D. et al. (2014). Diagnostic performance of ID Screen MVV-CAEV Indirect Screening ELISA in identifying small ruminant lentiviruses-infected goats. Polish Journal of Veterinary Sciences. Vol. 17, N°3, 501-506.

MILK

  1. Potârniche A. V. et al. (2023). Serological testing of an equal-volume milk sample–a new method to estimate the seroprevalence of small ruminant lentivirus infection? BMC Veterinary Research, 19(1), 43.

SERUM AND MILK

  1. Potărniche A.V. et al. (2021). Diagnostic accuracy of three commercial immunoenzymatic assays for small ruminant lentivirus infection in goats performed on individual milk samples. Preventive Veterinary Medicine, 191, 105347.
  2. Adjadj N.R. et al. (2019). (Non-) Sense of milk testing in small ruminant Lentivirus control programs in goats. Comparative Analysis of Antibody Detection and Molecular Diagnosis in Blood and Milk. Viruses, 12(1), 3.

SHEEP (SERUM)

  1. Pavlak M. et al. (2022). Seroprevalence and risk factors associated with Maedi Visna virus in sheep population in southwestern Croatia. Veterinarski arhiv, 92(3), 277-289.
  2. Jerre A. et al. (2022). Evaluation of three commercial ELISA tests for serological detection of maedi-visna virus using Bayesian latent class analysis. Preventive Veterinary Medicine, 208, 105765.
  3. Al-Baroodi S.Y. et al. (2022). Detection of Maedi-Visna virus in sheep in Nineveh province. Iraqi Journal of Veterinary Sciences, 36(1), 61-64.
  4. Alemnew E. et al. (2021). Serological Surveys Of Maedi-Visna Virus In Sheep Population Of Selected Areas Of Eastern Amhara, Ethiopia. Biomedicine and Nursing 2021;7(1): 59-64
  5. Alemnew E. et al. (2021). Seroprevalence and associated risk factors of Maedi-Visna in sheep population of North Shoa zone, Ethiopia. Research in: Agricultural & Veterinary SciencesVol.5, No.1, pp.11-18.
  6. Gezer T. et al. (2021). Investigation of Seroprevalence of Maedi-Visna Infection in some Sheep Flocks in Kars Province, Turkey. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 14(1), 48-51.
  7. Mosa A.H. et al. (2020). Serological and histopathological detection of Maedi-Visna virus in middle Iraq regions. Plant Arch, 20(2), 6339-6343.
  8. Yizengaw L. et al. (2020). Sero-prevalence and associated risk factors of Maedi-Visna virus in sheep population of selected area of Eastern Amhara, Ethiopia. Indian J. Anim. Hlth, 59(2), 150-158.
  9. Comtet L. et al. (2010). Validation of the ID SCREEN® Visna Maedi Indirect ELISA: specificity on BTV8-vaccinated sheep and detection of seroconversion. Poster presented at the 2010 EAVLD Meeting (Lelystad, Netherlands).

SHEEP AND GOATS (SERUM)

  1. Hailat N.Q. et al. (2022). Pathological, molecular, and serological study of small ruminant lentiviruses in Jordan. Veterinary World, 15(6), 1423.
  2. Michiels R. et al. (2021). Species-specific humoral immune responses in sheep and goats upon small ruminant lentivirus infections inversely correlate with protection against virus replication and pathological lesions. International Journal of Molecular Sciences, 22(18), 9824.
  3. Aalberts M. et al. (2021). Evaluation of five SRLV ELISAs for fitness for purpose in sheep and goat accreditation schemes in the Netherlands. Small Ruminant Research, 202, 106452.
  4. Savić S. et al. (2020). Prevalence of Small Ruminant Lentivirus Infections In Sheep and Goats in Some Regions of Vojvodina Province. Arhiv veterinarske medicine, Vol. 13, No. 1, 29 – 40.
  5. Olech M. et al. (2019). Molecular analysis of small-ruminant lentiviruses in Polish flocks reveals the existence of a novel subtype in sheep. Archives of Virology, 164(4), 1193-1198.
  6. Michiels R. et al. (2018). Comparative analysis of different serological and molecular tests for the detection of small ruminant lentiviruses (Srlvs) in Belgian sheep and goats. Viruses, 10(12), 696.

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