ID Screen® Influenza subtype-specific kits

Range of competitive ELISA kits for the detection of subtype-specific antibodies in avian serum or plasma.

  • May be used for DIVA diagnostics (Differentiation of Infected and Vaccinated Animals)*
  • Applicable to both domestic & wild avian species
  • High sensitivity and specificity: validated by OIE labs, with excellent results (1)
  • Easy-to-use, with results in under 2.5 hours
  • Comprehensive data analysis with the IDSoft™program
  • Positive and negative reference sera available as internal controls

* For example, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain, but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype as evidence of natural infection.  

Name Product code
ID Screen® Influenza H5 Antibody Competition FLUACH5-2P
FLUACH5-5P
ID Screen® Influenza H7 Antibody Competition FLUACH7-2P
FLUACH7-5P
ID Screen® Influenza H9 Antibody Competition FLUACH9-2P
FLUACH9-5P
ID Screen® Influenza N1 Antibody Competition FLUACN1-2P
FLUACN1-5P
ID Screen® Influenza N2 Antibody Competition FLUACN2-2P
FLUACN2-5P
ID Screen® Influenza N9 Antibody Competition FLUACN9-2P

 

Specifications
Method Competitive ELISA
Species Domestic and wild avian species
Specimens Serum or plasma
Coated antigen Purified native inactivated subtype-specific antigen
Conjugate Anti-subtype-HRP Mab conjugate (concentrated 10X)
Ordering information
Product code See above See above
Kit format 2 plates 5 plates
Reactions 192 480
Plate format 12 x 8-well strips 12 x 8-well strips
References
  1. Dundon, W.G,. Terregino, C., Tuttoilmondo, V., Pizzuto, M., Busani, L., Mancin, M., Cattoli, G., Capua, I. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Poster presented at EPIZONE 2007, Poland.
  2. Bötcher, L., Schöttker-Wegner, H.H., Nöckler, A. Summary of the German presentation at the AVID Meeting (September 15-17, 2010). Use of an H5-antibody ELISA for the serological differentiation of AIV-antibodies. Niedersächsisches Landesamt für Verbrauchschutz und Lebensmittelsicherheit Veterinärinstitut, Oldenburg, Allemagne.
  3. Robles, F. et al.  Implementation of  « DIVA » Concept with specific ELISA kits ; When Subunit H5 Avian Influenza Vaccine is Used. World Academy of Science, Engineering and Technology 65 2010.
Associated reference sera
Influenza A/N1 positive freeze-dried serum
Product code MRI-FLUACA/N1
Format 1 ml vial (freeze-dried)
Description

Freeze-dried positive chicken serum to be used as internal reference material for quality control.

Influenza H7 positive freeze-dried serum
Product code MRI-FLUH7
Format 0.5 ml vial (freeze-dried)
Description

Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serum
Product code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description

Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.