ID Screen® Influenza subtype-specific kits

Range of competitive ELISA kits for the detection of subtype-specific antibodies in avian serum or plasma.

  • May be used for DIVA diagnostics (Differentiation of Infected and Vaccinated Animals)*
  • Applicable to both domestic & wild avian species
  • High sensitivity and specificity: validated by OIE labs, with excellent results (1)
  • Easy-to-use, with results in under 2.5 hours
  • Comprehensive data analysis with the IDSoft™program
  • Positive and negative reference sera available as internal controls

* For example, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain, but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype as evidence of natural infection.  

Name Product code
ID Screen® Influenza H5 Antibody Competition FLUACH5-2P
FLUACH5-5P
ID Screen® Influenza H7 Antibody Competition FLUACH7-2P
FLUACH7-5P
ID Screen® Influenza H9 Antibody Competition FLUACH9-2P
FLUACH9-5P
ID Screen® Influenza N1 Antibody Competition FLUACN1-2P
FLUACN1-5P
ID Screen® Influenza N2 Antibody Competition FLUACN2-2P
FLUACN2-5P
ID Screen® Influenza N9 Antibody Competition FLUACN9-2P

 

Specifications
Method Competitive ELISA
Species Domestic and wild avian species
Specimens Serum or plasma
Coated antigen Purified native inactivated subtype-specific antigen
Conjugate Anti-subtype-HRP Mab conjugate (concentrated 10X)
Ordering information
Product code See above See above
Kit format 2 plates 5 plates
Reactions 192 480
Plate format 12 x 8-well strips 12 x 8-well strips
References
  1. Dundon, W.G,. Terregino, C., Tuttoilmondo, V., Pizzuto, M., Busani, L., Mancin, M., Cattoli, G., Capua, I. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Poster presented at EPIZONE 2007, Poland.
  2. Bötcher, L., Schöttker-Wegner, H.H., Nöckler, A. Summary of the German presentation at the AVID Meeting (September 15-17, 2010). Use of an H5-antibody ELISA for the serological differentiation of AIV-antibodies. Niedersächsisches Landesamt für Verbrauchschutz und Lebensmittelsicherheit Veterinärinstitut, Oldenburg, Allemagne.
  3. Robles, F. et al.  Implementation of  « DIVA » Concept with specific ELISA kits ; When Subunit H5 Avian Influenza Vaccine is Used. World Academy of Science, Engineering and Technology 65 2010.
Associated reference sera
Influenza H7 positive freeze-dried serum
Product code MRI-FLUH7
Format 0.5 ml vial (freeze-dried)
Description

Freeze-dried positive chicken serum to be used as internal reference material for quality control.

Influenza A/N1 positive freeze-dried serum
Product code MRI-FLUACA/N1
Format 1 ml vial (freeze-dried)
Description

Freeze-dried positive chicken serum to be used as internal reference material for quality control.

Ready-to-use chicken negative serum
Product code MRINEG-BIRD-RTU
Format 5 ml vial
Description

Ready to use specific pathogen free (SPF) chicken serum pool. Does not contain any antibodies against any avian pathogen. For use with all the IDvet poultry ELISA kits or for RSA testing.

SPF negative freeze-dried serum
Product code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description

Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

May be used for ELISA and RSA testing.