ID Screen® Influenza A Antibody Competition Multi-species

Competitive ELISA kit for the detection of anti-nucleoprotein (NP) antibodies.

Detects antibodies against all Influenza A subtypes and antigenic variants thanks to the use of a monoclonal antibody against a highly conserved epitope of the Influenza A virus nucleoprotein.

 

  • Highly sensitive and specific
  • Convenient multi-species test applicable to birds, horses, swine and others 
  • Easy-to-use, with results in under 2 hours
  • Comprehensive data analysis with the IDSoft™program
  • Positive and negative reference sera available as internal controls
  • For avian species: may be used with egg yolk samples which are easier to collect and cause less stress to the layers
Specifications
Method Competitive ELISA
Species Multiple species, including horses, birds, swine and others
Specimens Serum, plasma or egg yolk
Coated antigen Influenza A nucleoprotein (NP)
Conjugate Anti-NP-HRP conjugate (concentrated 10X)
Ordering information
Product code FLUACA-2P FLUACA-5P FLUACA-10P
Kit format 2 plates 5 plates 10 plates
Reactions 192 480 960
Plate format 12 x 8-well strips 12 x 8-well strips 12 x 8-well strips
References
Horses:
  1. Kittelberger, R., McFadden, A.M.J., Hannah, M.J., Jenner, J., Bueno, R., Vait, J., Kirkland, P.D., Delbridge, G., Heine, H.G., Selleck, P.W., Pearce, T.W., Pigott, C.J., O’Keefe, J.S., Comparative evaluation of four competitive/blocking ELISAs for the detection of influenza A antibodies in horses, Veterinary Microbiology (2010), doi:10.1016/ j.vetmic. 2010.08.014.
Birds:
  1. Bertran, K. et al. Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa). Veterinary Research 2011, 42:24.
  2. Dundon, W.G., Terregino, C., Tuttoilmondo V., Pizzuto, M., Busani L., Mancin, M., Cattoli, G., Capua I.. Preliminary validation of a commercial Avian Influenza N1 antibody competitive Elisa kit that can be used as part of a Diva strategy. Epizone 2007, Poland.
  3. Molia, S. et al. Avian influenza in backyard poultry of the Mopti region, Mali. Trop Anim Health Prod. 2009.
  4. Terregino, C. Evaluation of sensitivity and specificity of a commercial competitive avian influenza type A antibody ELISA kit, the OIE-FAO and National Reference Laboratory for Newcastle Disease and Avian Influenza, IZS delle Venezie, Legnaro (Padova), Italy.
Swine :
  1. Busquets, N. et al. Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus. Vet. Res (2010) 41:74.
  2. Lange, E. et al. Pathogenesis and transmission of the novel swine-origin influenza virus A/H1N1 after experimental infection of pigs. Journal of General Virology (2009), 90, 2119-2123.
Dogs:
  1. De Benedictis, P. et al. A diagnostic algorithm for detection of antibodies to influenza A viruses in dogs in Italy (2006-2008). J. Vet Diagn Invest 22:914-920 (2010).
Associated reference sera
Ready-to-use chicken negative serum
Product code MRINEG-BIRD-RTU
Format 5 ml vial
Description

Ready to use specific pathogen free (SPF) chicken serum pool. Does not contain any antibodies against any avian pathogen. For use with all the IDvet poultry ELISA kits or for RSA testing.

Influenza A/N1 positive freeze-dried serum
Product code MRI-FLUACA/N1
Format 1 ml vial (freeze-dried)
Description

Freeze-dried positive chicken serum to be used as internal reference material for quality control.

SPF negative freeze-dried serum
Product code MRINEG-BIRD
Format 0.5 ml vial (freeze-dried)
Description

Freeze-dried SPF negative chicken serum to be used as internal reference material for quality control.

May be used for ELISA and RSA testing.