Veterinary Diagnostics

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Brucella abortus / B. melitensis / B. suis

ID Screen® Brucellosis Serum Indirect Multi-species

ELISA

Indirect ELISA for the detection of antibodies against Brucella abortus, melitensis or suis in bovine, ovine, caprine and porcine serum and plasma (individual samples or pools of up to 10)

Advantages

Specifications

Format

References

Downloads

  • Reliable
    • Calibrated according to OIE specifications and Annex C of the European directive CEE 64/432 to correctly detect the OIEELISAspISS standard serum
  • Flexible
    • Multi-species test for ruminants and swine
  • Straightforward and versatile
    • Short and overnight incubations
    • Test individual serum or plasma samples, or pools of up to 10

Method :

Indirect ELISA

Species :

Bovine, ovine, caprine and porcine. Please contact IDvet for use in other species.

Specimens :

Individual serum or plasma samples, or pools of up to 10 samples.

Coated antigen :

Purified Brucella LPS

Conjugate :

Anti-multi-species-IgG-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

BRUS-MS-5P 5 plates 480 12 x 8-well strips
BRUS-MS-10P 10 plates 960 12 x 8-well strips

BOVINE

CATTLE

  1. Akoko J. M. et al. (2023). Mapping brucellosis risk in Kenya and its implications for control strategies in sub-Saharan Africa. Scientific Reports, 13(1), 20192.
  2. Ansel S. et al. (2023). Seroprevalence of Brucella spp and related risk factors in cattle from Laghouat District, South Algeria. Veterinaria,72(1), 37-43.
  3. Dheyab A. Q. et al. (2023). Seroprevalence of bovine brucellosis and its incidence in human in Thi-Qar province. Iraqi Journal of Veterinary Sciences, 37(4), 877-884.
  4. Sokun K. et al. (2023). Seroprevalence of Brucellosis Disease and Lumpy Skin Disease on Cattles at Svay Rieng and Prey Veng Province, Cambodia. Journal of Environmental Science and Engineering B 12 (2023) 198-205.
  5. Ntivuguruzwa J.B. et al. (2022). Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda. PLoS ONE 17(11): e0261595.
  6. Takanouo D. T. et al. (2022). Seroprevalence of bovine brucellosis in central Cameroon. The Bioscientist Journal, 10(3), 357-366.
  7. Kaba S. et al. (2021). Brucellosis Seroprevalence and Potential Transmission Risk to Workers at the Port-Bouët Abattoir, Abidjan, Côte d’Ivoire. Sumerianz Journal of Agriculture and Veterinary, 2021, Vol. 4, No. 3, pp. 76-84.
  8. Merga Sima D. et al. (2021). Seroprevalence of Bovine Brucellosis and Associated Risk Factors in Western Ethiopia. Veterinary Medicine: Research and Reports, 317-324.
  9. Ntivuguruzwa J. B. et al. (2020). Seroprevalence and associated risk factors of bovine brucellosis at the wildlife-livestock-human interface in Rwanda. Microorganisms, 8(10), 1553.
  10. Awah-Ndukum J., Mouiche M. M. M., Bayang, H. N. et al. (2018). Seroprevalence and associated risk factors of brucellosis among indigenous cattle in the Adamawa and north regions of Cameroon. Veterinary Medicine International, 2018.
  11. Awah-Ndukum J., Mouiche, M. M. M., Kouonmo-Ngnoyum L. et al. (2018). Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaoundéré, Cameroon. BMC infectious diseases, 18(1), 1-13

CATTLE AND BUFFALOES

  1. Jadav S. J. et al. (2022). Seroprevalence of Bovine Brucellosis in Panchmahals and Mahisagar Districts of Gujarat State of India. Indian Journal of Veterinary Sciences & Biotechnology, 18(2), 141-143.
  2. Khan M.R. et al. (2021). Seroprevalence and Associated Risk Factors of Bovine Brucellosis in District Gujranwala, Punjab, Pakistan. Animals, 11, 174.
  3. Siengsanan-Lamont J. et al. (2021).The Development of an Abattoir-Based Surveillance System in Lao PDR for the Detection of Zoonoses in Large Ruminants: Q Fever and Brucellosis Seroepidemiology as a Pilot Study. Animals, 11, 742.
  4. Jamil T. et al. (2020). Serological and molecular detection of bovine brucellosis at institutional livestock farms in Punjab, Pakistan. International journal of environmental research and public health, 17(4), 1412.

SMALL RUMINANTS

  1. Dadar M. and Alamian S. (2020). Investigation of small ruminant brucellosis among smallholder farms: The missing link in control programmes of endemic areas. Zoonoses and Public Health, 68(5), 376-383.
  2. Ebid M. et al. (2020). Seroprevalence of brucellosis in sheep and goats in the Arabian Gulf region. Veterinary World, 13(8), 1495.
  3. Ullah Q. et al. (2020). Epidemiology and Associated Risk Factors for Brucellosis in Small Ruminants Kept at Institutional Livestock Farms in Punjab, Pakistan. Front. Vet. Sci. 7:526.
  4. Bait K. S. et al. (2019). Sero-prevalence of brucellosis in small ruminants of Western Maharashtra. Journal of Entomology and Zoology Studies; 7(5): 1417-1420.
  5. Burns R. J. L. et al (2018). Serosurveillance of Coxiellosis (Q-fever) and Brucellosis in goats in selected provinces of Lao People’s Democratic Republic. PLoS neglected tropical diseases, 12(4), e0006411.
  6. Ijale G. O. et al. (2014). Determination of risk factors and level of awareness of caprine brucellosis amongst goat owners in Oju, Benue state, Nigeria. Animal Health and Production, 62, 177.
  7. Kandeel A. E. et al. (2014). Seroprevalence of Brucellosis within sheep and goat flocks in Alkamil province in Saudi Arabia. Bothalia J, 44(5), 131-138.

BOVINE AND SMALL RUMINANTS

  1. Getachew S. et al. (2023). Seroprevalence of Brucella infection in cattle and small ruminants in South Omo zone, southern Ethiopia. Ethiopian Veterinary Journal, 27(2), 125-144.
  2. Ismail A. H. et al. (2022). Comparative study on Seroprevalence and Associated Risk factors of Brucella Infection among sheep, goat and cattle in Somalia. Preprint Research square; https://doi.org/10.21203/rs.3.rs-1951554/v1
  3. Troupin C. et al. (2022). Seroprevalence of brucellosis, Q fever and Rift Valley fever in domestic ruminants in Guinea in 2017–2019. BMC Veterinary Research, 18(1), 64.
  4. Hassan-Kadle A. et al. (2021). Rift Valley fever and Brucella spp. in ruminants, Somalia. BMC Veterinary Research, 17(1), 1-6.
  5. Ukwueze K. O. et al. (2020). Seroprevalence of brucellosis and associated factors among livestock slaughtered in Oko-Oba abattoir, Lagos State, southwestern Nigeria. Pan African Medical Journal, 36(1).
  6. Barkallah M. et al. (2017). A mixed methods study of ruminant brucellosis in central-eastern Tunisia. Tropical animal health and production, 49, 39-45.
  7. Elandalousi R. B. et al. (2015). Séroprévalence des maladies abortives zoonotiques chez les ruminants au nord de la Tunisie. Research fr, 2, 1419.

CAMELS

  1. Benfodil K. et al. (2022). Seroprevalence and associated risk factors for camel brucellosis in south Algeria. Veterinaria, 71(1), 17-26.
  2. Muturi M. et al. (2021). Serological evidence of single and mixed infections of Rift Valley fever virus, Brucella spp. and Coxiella burnetii in dromedary camels in Kenya. PLoS Negl Trop Dis 15(3): e0009275.
  3. Tanimoun H. M. et al. (2021). Prevalence and risk behaviours of camel brucellosis transmission in the peri-urban dairy basin of Niamey, Niger. International Journal of Biological and Chemical Sciences, 15(2), 379-387.
  4. Khan A. U. et al. (2020). Seroprevalence and molecular identification of Brucella spp. in camels in Egypt. Microorganisms, 8(7), 1035.
  5. Shabbir M. Z. et al. (2020). Sentinel surveillance of selected veterinary and public health pathogens in camel population originating from Southern Punjab province, Pakistan. Acta tropica, 205, 105435.

SWINE

  1. Awah-Ndukum J. et al. (2018). Serological survey and associated risk factors of brucellosis in pigs in Cameroon. Bulletin of Animal Health and Production in Africa 66(4):785-801.
  2. Pokhrel K. et al. (2021). Seroprevalence of Brucellosis among Pigs of Commercial Farms in Chitwan District of Nepal. Tribhuvan University Journal of Microbiology 8(1): 79-82.
  3. Tshilenge M.G. et al. (2020). Occurrence of brucellosis in pigs kept in confinement and free ranging systems in the Democratic Republic of the Congo. International Journal of Veterinary Sciences and Animal Husbandry 2020; 5(6): 39-45.

EQUINE

  1. Nematy Oozee Y. et al. (2023). Serological prevalence of Brucellosis in horses in the suburb of Tabriz, Iran. Journal of Zoonotic Diseases.
  2. Adamu S. G. et al. (2020). Seroprevalence of Brucella antibodies in Donkeys (Equus asinus) in Yobe south senatorial zone, Northeastern Nigeria. Journal of Equine Science, 31(1), 5-10.
  3. Hussain A. et al. (2020). Serological and molecular investigation of brucellosis in breeding equids in Pakistani Punjab. Pathogens, 9(9), 673.

DOGS

  1. Marami L. M. et al (2021). Seroprevalence and associated risk factors of canine leptospira and Brucella species infection in west Shewa zone, Central Ethiopia. Veterinary Medicine: Research and Reports, 33-42.
  2. Jamil T. et al. (2019). Serological and molecular investigation of Brucella species in dogs in Pakistan. Pathogens, 8(4), 294.

WILDLIFE

  1. Gakuya F. et al. (2022). Evidence of co-exposure with Brucella spp, Coxiella burnetii, and Rift Valley fever virus among various species of wildlife in Kenya. PLoS Negl Trop Dis 16(8): e0010596.
  2. Macías Luaces L. et al. (2023). Infection in Wild Boars (Sus scrofa) of Bavaria, Germany, 2019 to 2021 and Associated Genome Analysis of Five B. suis Biovar 2 Isolates. Microorganisms 2023, 11, 478.
  3. Fredriksson-Ahomaa M. et al. (2020). Foodborne zoonoses common in hunted wild boars. Ecohealth, 17, 512-522.
  4. Wu J. Y. et al. (2020). Seroprevalence of five zoonotic pathogens in wild ruminants in Xinjiang, Northwest China. Vector-Borne and Zoonotic Diseases, 20(12), 882-887.

MULTISPECIES

  1. Kamga R. M. et al. (2020). Detection of Brucella antibodies in domestic animals of southern Cameroon: Implications for the control of brucellosis. Veterinary Medicine and Science, 6(3), 410-420.
  2. Singh U.M. et al. (2016). Seroprevalence of brucellosis during pre-monsoon and post monsoon seasons in different farm animal species of Nepal. Nepalese Vet J, 32(1), 1-6.

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