Veterinary Diagnostics

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Bluetongue virus (BTV)

ID Screen® Bluetongue Competition

ELISA

Competitive ELISA kit for the detection of anti-VP7 antibodies in serum or plasma from multiple species

Advantages

Specifications

Format

References

Downloads

  • Proven specificity and sensitivity and widespread use in recent outbreaks
  • Detection of antibodies against all BTV serotypes, at least 5-8 days after natural infection, thanks to the use of a monoclonal antibody against the highly conserved VP7 protein
  • Easy-to-use : only one wash step, with results in 90 minutes

Method :

Competitive ELISA

Species :

Multiple species, including sheep, goats, cattle, buffalo, deer, and others

Specimens :

Serum or plasma

Coated antigen :

Recombinant VP7

Conjugate :

Anti-VP7-HRP conjugate (concentrated 10X)

Product reference

Kit format

Reactions

Plate format

BTC-5P 5 plates 480 12 x 8-well strips
BTC-10P 10 plates 960 12 x 8-well strips

BTV ANTIBODY DETECTION (UNIDENTIFIED SEROTYPES)

CATTLE AND SMALL RUMINANTS

  1. Puri B. et al. (2022). Seroprevalence of bluetongue disease among domestic ruminants raised in International Border Areas of Nepal. Sarhad Journal of Agriculture, 38(2), 555-562.
  2. Douangngeun B. et al. (2016). Seroprevalence of Q fever, brucellosis, and bluetongue in selected provinces in Lao People’s Democratic Republic. The American journal of tropical medicine and hygiene, 95(3), 558.

SMALL RUMINANTS

  1. Daif S. et al. (2022). Serological and molecular prevalence study of bluetongue virus in small domestic ruminants in Morocco. Scientific Reports, 12(1), 1-11.
  2. Haile T. et al. (2022). Seroprevalence of Bluetongue Virus Antibodies in Ovine in Maji District of West Omo Zone, Southwest Ethiopia. Veterinary Medicine: Research and Reports, 13, 257-264.
  3. Munmun T. K. et al. (2022). Seroprevalence and risk factors of bluetongue virus in sheep of Chattogram, Bangladesh. Veterinary World, 15(6), 1589.
  4. Abera T. et al. (2018). Bluetongue disease in small ruminants in southwestern Ethiopia: cross-sectional sero-epidemiological study. BMC Research Notes, 11(1), 1-6.
  5. Malik A. I. et al. (2018). Sero-epidemiology of bluetongue virus (BTV) infection in sheep and goats of Khyber Pakhtunkhwa province of Pakistan. Acta Tropica, 182, 207-211.

BTV ANTIBODY DETECTION (MULTI SEROTYPES)

CATTLE

  1. Dommergues L. et al. (2019). Evidence of bluetongue and Epizootic Haemorrhagic disease circulation on the island of Mayotte. Acta Tropica, 191, 24-28.
  2. Viarouge C. et al. (2014). Identification of bluetongue virus and epizootic hemorrhagic disease virus serotypes in French Guiana in 2011 and 2012. Veterinary Microbiology, 174(1-2), 78-85.

CATTLE AND SMALL RUMINANTS

  1. Chambaro H. M. et al. (2020). Co-Circulation of Multiple Serotypes of Bluetongue Virus in Zambia. Viruses, 12(9), 963.
  2. Batten C. A. et al. (2008). Bluetongue virus: European Community inter-laboratory comparison tests to evaluate ELISA and RT-PCR detection methods. Veterinary Microbiology, 129(1-2), 80-88.

SMALL RUMINANTS

  1. Sohail T. et al. (2018). Seroprevalence of Bluetongue Virus in small ruminants in Balochistan province, Pakistan. Transboundary and emerging diseases, 65(5), 1272-1281.
  2. Kamar D. et al. (2015). Bluetongue virus in Morocco from 2004-2012. J. Anim. Health Prod, 3(3), 48-53.

CAMELS

  1. Shabbir M. Z. et al. (2020). Sentinel surveillance of selected veterinary and public health pathogens in camel population originating from Southern Punjab province, Pakistan. Acta Tropica, 205, 105435.

BTV1 ANTIBODY DETECTION

  1. Corbière F. et al. (2012). Bluetongue virus serotype 1 in wild ruminants, France, 2008–10. Journal of wildlife diseases, 48(4), 1047-1051.
  2. Meyer G. et al. (2009). Lethal bluetongue virus serotype 1 infection in llamas. Emerging infectious diseases, 15(4), 608-610.

BTV3 ANTIBODY DETECTION

  1. Holwerda M. et al. (2023). Emergence of bluetongue virus serotype 3 in the Netherlands in September 2023. bioRxiv (2023): 2023-09.
  2. Ahmed S. et al. (2019). Presence of bluetongue and epizootic hemorrhagic disease viruses in Egypt in 2016 and 2017. Infection, Genetics and Evolution, 73, 221-226.

BTV4 ANTIBODY DETECTION

  1. Sailleau C. et al. (2018). Complete genome sequence of bluetongue virus serotype 4 that emerged on the French island of Corsica in December 2016. Transboundary and emerging diseases, 65(1), e194-e197.
  2. Katsoulos P. D. et al. (2016). Epidemiological characteristics and clinicopathological features of bluetongue in sheep and cattle, during the 2014 BTV serotype 4 incursion in Greece. Tropical Animal Health and Production, 48(3), 469-477.

BTV8 ANTIBODY DETECTION

CATTLE

  1. Bournez L. et al. (2018). Estimation of French cattle herd immunity against bluetongue serotype 8 at the time of its re-emergence in 2015. BMC Veterinary Research, 14(1), 1-11.
  2. Courtejoie N. et al. (2018). Circulation of bluetongue virus 8 in French cattle, before and after the re‐emergence in 2015. Transboundary and emerging diseases, 65(1), 281-284.
  3. Vitale N. et al. (2016). Factors Affecting Seroconversion Rates in Cattle Vaccinated with Two Commercial Inactivated BTV‐8 Vaccines Under Field Conditions. Transboundary and emerging diseases, 63(2), 175-183.
  4. Niedbalski W. (2011). Evaluation of commercial ELISA kits for the detection of antibodies against bluetongue virus. Polish Journal of Veterinary Sciences.

SMALL RUMINANTS

  1. Hilke J. et al. (2019). Presence of Antibodies against Bluetongue Virus (BTV) in Sheep 5 to 7.5 Years after Vaccination with Inactivated BTV-8 Vaccines. Viruses, 11(6), 533.

CATTLE AND SMALL RUMINANTS

  1. Toussaint J. F. et al. (2007). Bluetongue in Belgium, 2006. Emerging Infectious Diseases, 13(4), 614.

CAMELIDS

  1. Schulz C. et al. (2012). Experimental infection of South American camelids with bluetongue virus serotype 8. Veterinary Microbiology, 154(3-4), 257-265.

WILDLIFE

  1. Jauniaux T. P. et al. (2008). Bluetongue in Eurasian lynx. Emerging Infectious diseases, 14(9), 1496.

BTV14 ANTIBODY DETECTION

  1. Koltsov A. et al. (2020). Identification and characterization of Bluetongue virus serotype 14 in Russia. Frontiers in Veterinary Science, 7, 26.

ATYPICAL BTV (BTV25-26-27-28)

  1. Sana K. et al. (2022). Risk‐based serological survey of bluetongue and the first evidence of bluetongue virus serotype 26 circulation in Tunisia. Veterinary Medicine and Science.
  2. Bumbarov V. et al. (2020). Characterization of bluetongue virus serotype 28. Transboundary and emerging diseases, 67(1), 171-182.
  3. Ries C. et al. (2020). Isolation and cultivation of a new isolate of BTV-25 and presumptive evidence for a potential persistent infection in healthy goats. Viruses, 12(9), 983.
  4. Bréard E. et al. (2018). Bluetongue virus serotype 27: Experimental infection of goats, sheep and cattle with three BTV‐27 variants reveal atypical characteristics and likely direct contact transmission BTV‐27 between goats. Transboundary and emerging diseases, 65(2), e251-e263.

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