ID Screen® Akabane Competition

Competitive ELISA for the detection of antibodies against AKAV in ruminant serum or plasma.

  • High correlation with the virus neutralisation test (VNT)
  • Sensitive and specific, with clear separation of positive and negative results
  • No cross-reactions with SBV (2)
  • Fast and convenient : ready-to-use components, with results in just over 2 hours
  • Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
  • Cost-effective for the analysis of large numbers of samples, notably in comparison with VNT
Specifications
Method Competitive ELISA
Species Ruminants
Specimens Serum and plasma
Coated antigen Purified virus
Conjugate Anti-G1-HRP conjugate (concentrated 10X)
Ordering information
Product code AKAC-4P AKAC-10P
Kit format 4 plates 10 plates
Reactions 384 960
Plate format 12 x 8-well strips 12 x 8-well strips
References
  1. Lunt, RA, White, JR, Newberry, KM, (CSIRO Livestock Industries, Geelong, Australia), Pourquier, P & Comtet, L (IDvet, Montpellier, France). Development of a commercial ELISA for the detection of serum antibody to Akabane virus. Poster presented at the 2007 WAVLD meeting, Melbourne, Australia.
  2. Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the 2013 Epizone Congress, Brussels, Belgium.
Associated reference sera
Akabane positive freeze-dried serum
Product code MRI-AKA
Format 1 ml vial (freeze-dried)
Description

Positive freeze-dried ovine serum to be used as internal reference material for quality control.

Negative bovine freeze-dried serum
Product code MRINEG-BOV
Format 1 ml vial (freeze-dried)
Description

Negative freeze-dried bovine serum to be used as internal reference material for quality control.