Competitive ELISA for the detection of antibodies against AKAV in ruminant serum or plasma.

• High correlation with the virus neutralisation test (VNT)
• Sensitive and specific, with clear separation of positive and negative results
• No cross-reactions with SBV (2)
• Fast and convenient : ready-to-use components, with results in just over 2 hours
• Practical: you can easily check that all samples have been correctly deposited because the dilution buffer changes colour upon addition of the sera
• Cost-effective for the analysis of large numbers of samples, notably in comparison with VNT

Specifications
Method	Competitive ELISA
Species	Ruminants
Specimens	Serum and plasma
Coated antigen	Purified virus
Conjugate	Anti-G1-HRP conjugate (concentrated 10X)

Ordering information
Product code	AKAC-4P	AKAC-10P
Kit format	4 plates	10 plates
Reactions	384	960
Plate format	12 x 8-well strips	12 x 8-well strips

References

Lunt, RA, White, JR, Newberry, KM, (CSIRO Livestock Industries, Geelong, Australia), Pourquier, P & Comtet, L (IDvet, Montpellier, France). Development of a commercial ELISA for the detection of serum antibody to Akabane virus. Poster presented at the 2007 WAVLD meeting, Melbourne, Australia. Comtet L. & Pourquier P. (IDvet, France). ID Screen® Schmallenberg virus Competition Multi-species: validation of a competitive ELISA for the detection of antibodies directed against the Schmallenberg virus nucleoprotein. Poster presented at the 2013 Epizone Congress, Brussels, Belgium.