Product listReal-time RT-PCR for the relative qualitative or quantitative detection of Coxiella burnetii
This relative qualitative or quantitative triplex test simultaneously amplifies target DNA of Coxiella burnetii, an endogenous internal control, and a non-target exogenous positive control.
It may be used to test ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples.
Features:
- Rapid 1 hour amplification protocol
- Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
- Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
- High performance: DLPCR< 10 copies / PCR and specificity of 100%.
- Validated by the OIE reference laboratory and according to the French standard NFU47-600-2 (underway)
- Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.
| Specifications | ||
|---|---|---|
| Product code | IDQF-50 | IDQF-100 |
| Reactions | 50 | 100 |
| Method | Real time RT PCR - Triplex - Relative Qualitative - Quantitative |
| Species | Ruminants |
| Sample types | Ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples |
| Associated extraction kits |
|---|
| ID Gene™ Spin Universal Extraction Kit | |
| Product code | SPIN50 / SPIN250 |
| Format | 50 preps / 250 preps |
| Description | Extraction system using silica columns for all matrices and all veterinary pathogens |
| ID Gene™ Mag Fast Extraction Kit | |
| Product code | MAGFAST384 |
| Format | 384 preps |
| Description | Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).
⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!
⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 90 minutes (extraction and amplification)
⇒ This kit is compatible with the following automated extraction systems:
|
| ID Gene™ Mag Universal Extraction Kit | |
| Product code | MAG192 / MAG384 |
| Format | 192 preps / 384 preps |
| Description | Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA).
⇒ Results in 40 min ⇒ This kit is compatible with the following automated extraction systems:
⇒ May be used with most commercial amplification kits (generally validated with this type of extraction protocol) |