Real-time RT-PCR for the qualitative or quantitative detection of Coxiella burnetii
This qualitative or quantitative triplex test simultaneously amplifies target DNA of Coxiella burnetii, an endogenous internal control, and a non-target exogenous positive control.
It may be used to test ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples.
Features:
- Rapid 1 hour amplification protocol
- Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
- Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
- High performance: DLPCR< 10 copies / PCR and specificity of 100%.
- Validated by the OIE reference laboratory and according to the French standard NFU47-600-2 (underway)
- Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.
Specifications | ||
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Product code | IDQF-50 | IDQF-100 |
Reactions | 50 | 100 |
Method | Real time RT PCR - Triplex - Qualitative - Quantitative |
Species | Ruminants |
Sample types | Ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples |
Associated products |
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ID Gene® Spin Universal Extraction Kit | |
Product code | SPIN50 / SPIN250 |
Format | 50 preps / 250 preps |
Description | Extraction system using silica columns for all matrices and all veterinary pathogens |
ID Gene™ Mag Fast Extraction Kit | |
Product code | MAGFAST384 |
Format | 384 preps |
Description | Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA). ⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min! ⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification) ⇒ Compatible with most open extraction robots for magnetic beads |
ID Gene® Mag Universal Extraction Kit | |
Product code | MAG384 |
Format | 384 preps |
Description | Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA).
⇒ Results in 40 min ⇒ Compatible with most open extraction robots for magnetic beads ⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol. |