ID Gene® Q Fever Triplex

Real-time RT-PCR for the qualitative or quantitative detection of Coxiella burnetii

This qualitative or quantitative triplex test simultaneously amplifies target DNA of Coxiella burnetii, an endogenous internal control, and a non-target exogenous positive control.

It may be used to test ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples.

 

Features:

  • Rapid 1 hour amplification protocol
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene® PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • High performance: DLPCR< 10 copies / PCR and specificity of 100%.
  • Validated by the OIE reference laboratory and according to the French standard NFU47-600-2 (underway)
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.
Specifications
Product code IDQF-50 IDQF-100
Reactions 50 100
Method Real time RT PCR - Triplex - Qualitative - Quantitative
Species Ruminants
Sample types Ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples
Associated extraction kits
ID Gene® Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene® Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene® Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.