ID Gene™ Q Fever-Chlamydophila spp

Real-time RT-PCR for the qualitative or quantitative detection of Coxiella burnetii and Chlamydophila spp.

This qualitative or quantitative triplex test simultaneously amplifies target DNA of Coxiella burnetii and Chlamydophila spp., and an endogenous internal control.

It may be used to test ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples.

 

Features:

  • Rapid 1 hour amplification protocol
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • High performance: DLPCR< 10 copies / PCR and specificity of 100%.
  • Validated by the OIE reference laboratory and according to the French standard NFU47-600-2 (underway)
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR
Specifications
Product code IDQFCH-50 IDQFCH-100
Reactions 50 100
Method Real-Time PCR - Triplex - Qualitative relative -
Quantitative (Q Fever only)
Species Ruminants
Sample types Ruminant swab supernatant (placental, vaginal, cervical), milk, spleen and gastric juice samples
Associated extraction kits
ID Gene™ Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene™ Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.