ID Gene® Peste des Petits Ruminants Duplex

ID Gene® Peste des Petits Ruminants (IDPPR) kit is a real-time RT-PCR (RT-qPCR) kit that amplifies a target sequence in Peste des Petits Ruminants (PPRV) viral genome.

This kit is a qualitative duplex test. It simultaneously amplifies target RNA and endogenous internal control.

 

  • The IDPPR qPCR is the ONLY commercial PPR qPCR to offer a calibrated target positive control (TPC) to validate the entire PCR testing process, from extraction to amplification:
    • VALIDATE EXTRACTION AND AMPLIFICATION OF THE TARGET
    • MONITOR VARIATIONS IN ANALYTICAL SENSITIVITY
  • The IDPPR qPCR is the ONLY commercial qPCR co-developed and validated by the FAO / OIE CIRAD laboratory
  • The IDPPR qPCR offers the FASTEST amplification protocol, with extraction and amplification in only 80 minutes: extraction in 20 minutes using the IDvet MAGFAST kit, and amplification in 60 minutes
  • The kit includes an endogenous internal control because the preferred sample type for this disease is ocular swabs. It is therefore important to validate sample presence.
Specifications
Product code IDPPR-50 IDPPR-100
Reactions 50 100
Method Real time RT-PCR - Duplex - Qualitative
Species Caprine and ovine
Sample types Whole blood, swabs (oral, ocular,nasal and rectal) organ or tissue
Associated extraction kits
ID Gene® Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene® Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene® Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.