Real-time PCR for the relative qualitative or quantitative detection of Mycobacterium avium subsp. paratuberculosis in ruminant faeces samples (individual samples or pools of up to 10)

This relative qualitative or quantitative duplex triplex test simultaneously amplifies a target sequence in the Mycobacterium avium subsp. paratuberculosis (Map) genome and an exogenous internal control.

 

It is the only commercial Paratuberculosis PCR to include:

• positive target and non-target controls* to be extracted along with field samples in order to evaluate extraction efficiency and confirm the absence of inhibitors in each well
• a target positive control calibrated at between 10 and 100 times the MDL (method detection limit) in order to monitor variations in analytical sensitivity.

Relative qualitative testing to determine culling priorities

Studies have shown that animals with greater than 3000 mycobacteria / g of faeces are more likely to be chronically infected, and therefore a potential source of disease spread. (Animals with less than 3000 mycobacteria / g of faeces are more likely to be passive carriers.)

As the target positive control is calibrated at 3000 myobacteria / g of faeces, samples with a Ct less than the TPC may be classified as chronically-infected, and higher than the TPC as passive carriers.  By identifying chronically infected animals in this way, the kit may be used as a decision-making tool to determine culling priorities in the herd.

 

Quantitative testing

For quantitative interpretation, the kit also includes synthetic target DNA titrated in copies / PCR and expressed as mycobacteria / gram of faeces.

 

EZPREP sample preparation kit

Facilitate sample preparation with the EZPREP kit: no weighing step required, and samples are processed in under two minutes using a special droplet bottle with an integrated filter!

 

Other features:

• High performance: DL_PCR< 20 copies / PCR and specificity of 100%.
• Rapid (1 hour) or standard (2 hour) amplification protocols
• Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
• Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
• Validated by the French national reference laboratory and according to the French standard NFU47-600-2.
• Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.

* Target Positive Control (TPC) included in the kit: supernatant of naturally-infected bovine faeces,

* Non-Target Positive Control (NTPC) included in the kit: culture supernatant of a non-pathogenic mycobacterium.

Specifications
Product code	IDMAP-50	IDMAP-100
Reactions	50	100

Method	Real time RT PCR - Duplex - Qualitative relative - Quantitative
Species	Ruminants
Sample types	Ruminant faeces (individual samples or pools of up to 10); culture and culture broth