ID Gene® Paratuberculosis Duplex

Real-time PCR for the relative qualitative or quantitative detection of Mycobacterium avium subsp. paratuberculosis in ruminant faeces samples (individual samples or pools of up to 10)

This relative qualitative or quantitative duplex test simultaneously amplifies a target sequence in the Mycobacterium avium subsp. paratuberculosis (Map) genome and an exogenous internal control.

 

It is the only commercial Paratuberculosis PCR to include:

  • positive target and non-target controls* to be extracted along with field samples in order to evaluate extraction efficiency and confirm the absence of inhibitors in each well
  • a target positive control calibrated at between 10 and 100 times the MDL (method detection limit) in order to monitor variations in analytical sensitivity.

Relative qualitative testing to determine culling priorities

Studies have shown that animals with greater than 3000 mycobacteria / g of faeces are more likely to be chronically infected, and therefore a potential source of disease spread. (Animals with less than 3000 mycobacteria / g of faeces are more likely to be passive carriers.)

As the target positive control is calibrated at 3000 myobacteria / g of faeces, samples with a Ct less than the TPC may be classified as chronically-infected, and higher than the TPC as passive carriers.  By identifying chronically infected animals in this way, the kit may be used as a decision-making tool to determine culling priorities in the herd.

 

Quantitative testing

For quantitative interpretation, the kit also includes synthetic target DNA titrated in copies / PCR and expressed as mycobacteria / gram of faeces.

 

EZPREP sample preparation kit

Facilitate sample preparation with the EZPREP kit: no weighing step required, and samples are processed in under two minutes using a special droplet bottle with an integrated filter!

 

Other features:

  • High performance: DLPCR< 20 copies / PCR and specificity of 100%.
  • Rapid (1 hour) or standard (2 hour) amplification protocols
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene® PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • Validated by the French national reference laboratory and according to the French standard NFU47-600-2.
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.

* Target Positive Control (TPC) included in the kit: supernatant of naturally-infected bovine faeces,

* Non-Target Positive Control (NTPC) included in the kit: non-pathogenic mycobacterium.

Specifications
Product code IDMAP-50 IDMAP-100
Reactions 50 100
Method Real time RT PCR - Duplex - Qualitative relative - Quantitative
Species Ruminants
Sample types Ruminant faeces (individual samples or pools of up to 10); culture and culture broth
Associated extraction kits
ID Gene® Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene® Mag Paratuberculosis Extraction Kit
Product code MAGMAP192
Format 192 preps
Description

Magnetic bead DNA extraction kit for Mycobacterium avium subsp. paratuberculosis for ruminant faeces samples  (individual samples or pools of up to 10).

⇒ Results in 40 min

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene® Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene® Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.
ID Gene® Easy preparation for faeces samples
Product code EZPREP
Format 100 EZ Drop bottles (preps)
Description

A simplified method for the preparation of faeces sample to be tested for Mycobacterium avium subsp. paratuberculosis and other pathogens.

 

⇒ Allows for pre-treatment of faeces samples without any weighing step

⇒ Use in combination with IDMAP: ID Gene™ Paratuberculosis Duplex (IDMAP) PCR kit

 

Benefits:

⇒ Rapid: process samples in only 2 minutes

⇒ Easy-to-use:

  • No weighing step
  • Easily open pools for re-test (conserve individual homogenates at -20°C)

⇒ Improved test sensitivity and reproducibility thanks to the production of homogenous faecal extracts