ID Gene™ Neospora Caninum Duplex

Real-time RT-PCR for the qualitative detection of Neospora caninum

 This qualitative duplex test simultaneously amplifies a target sequence in the Neospora caninum genome, and an endogenous internal control.

It may be used to analyse brain, placenta or muscle homogenates.

 

It is the only commercial N. caninum PCR to include a positive target control* to be extracted along with field samples in order to evaluate extraction, purification and amplification efficiency

 

Other features:

  • Rapid 1 hour amplification protocol
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • High performance: DLPCR< 10 copies / PCR and specificity of 100%.
  • Validated by the French national reference laboratory and according to the French standard NFU47-600-2
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR

* Target Positive Control (TPC) included in the kit: negative brain tissue spiked with inactivate N. caninum strain, and calibrated at between 10 and 100 times the detection limit of the method (MDL).  

Specifications
Product code IDNEO-50 IDNEO-100
Reactions 50 100
Method Real time RT PCR - Duplex - Qualitative
Species Ruminants
Sample types Brain, placenta, muscle
Associated extraction kits
ID Gene™ Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens

ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

 

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 90 minutes (extraction and amplification)

 

⇒ This kit is compatible with the following automated extraction systems:

  • MagMax™ Express-96 Magnetic Particles Processor (Thermo Scientific™)
  • KingFisher™ 96 (Thermo Scientific™)
  • KingFisher™ Flex (Thermo Scientific™)
  • KingFisher™ mL (Thermo Scientific™)
ID Gene™ Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

⇒ This kit is compatible with the following automated extraction systems:

  • MagMax™ Express-96 Magnetic Particles Processor (Thermo Scientific™)
  • KingFisher™ 96 (Thermo Scientific™)
  • KingFisher™ Flex (Thermo Scientific™)
  • KingFisher™ mL (Thermo Scientific™)

⇒ May be used with most commercial amplification kits (generally validated with this type of extraction protocol)