Real-time RT-PCR for the qualitative detection of Neospora caninum
This qualitative duplex test simultaneously amplifies a target sequence in the Neospora caninum genome, and an endogenous internal control.
It may be used to analyse brain, placenta or muscle homogenates.
It is the only commercial N. caninum PCR to include a positive target control* to be extracted along with field samples in order to evaluate extraction, purification and amplification efficiency
Other features:
- Rapid 1 hour amplification protocol
- Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
- Uses the same extraction and amplification protocols common to all ID Gene® PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
- High performance: DLPCR< 10 copies / PCR and specificity of 100%.
- Validated by the French national reference laboratory and according to the French standard NFU47-600-2
- Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR
* Target Positive Control (TPC) included in the kit: negative brain tissue spiked with inactivate N. caninum strain, and calibrated at between 10 and 100 times the detection limit of the method (MDL).
| Specifications | ||
|---|---|---|
| Product code | IDNEO-50 | IDNEO-100 |
| Reactions | 50 | 100 |
| Method | Real time RT PCR - Duplex - Qualitative |
| Species | Ruminants |
| Sample types | Brain, placenta, muscle |
| Associated products |
|---|
| ID Gene® Spin Universal Extraction Kit | |
| Product code | SPIN50 / SPIN250 |
| Format | 50 preps / 250 preps |
| Description | Extraction system using silica columns for all matrices and all veterinary pathogens |
| ID Gene® Mag Fast Extraction Kit | |
| Product code | MAGFAST384 |
| Format | 384 preps |
| Description | Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).
⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min! ⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification) ⇒ Compatible with the following automated extraction systems:
|
| ID Gene® Mag Universal Extraction Kit | |
| Product code | MAG192 / MAG384 |
| Format | 192 preps / 384 preps |
| Description | Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA).
⇒ Results in 40 min
⇒ Compatible with the following automated extraction systems:
⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol. |