ID Gene™ Mycobacterium tuberculosis complex Duplex

The ID Gene™ Mycobacterium tuberculosis complex Duplex (IDTUB) kit is a qPCR kit that amplifies a target sequence in the genome of members of the Mycobacterium tuberculosis complex (MTC).

This kit is a qualitative Duplex test. It simultaneously amplifies target DNA from member(s) of the MTC (M. tuberculosis, M. bovis, M.africanum, M. microti, M.canetti, M.caprae, and M.pinnipedii) and an exogenous internal control.

This kit may be used to test lymph nodes and peripheral tissues from cattle, sheep, goats, deers, suids and badgers.

 

  • The most sensitive PCR kit on the market (LDPCR = 1 copy/PCR and LDmethod = 300 UG/mL)
  • Developed according to the requirements of Terrestrial Animal Health Code (OIE) and the French National Reference Laboratory for Tuberculosis (ANSES)
  • Includes ready-to-use reagents, positive controls to evaluate the efficiency of the extraction and amplification steps, and an exogenous internal control
  • The fastest protocol on the market
  • Suitable for use with most extraction systems and all thermocyclers
Specifications
Product codeIDTUB-50IDTUB-100
Reactions50100
MethodqPCR - Duplex - Qualitative
SpeciesCattle, sheep, goats, deers, suids and badgers
Sample typesLymph nodes and peripheral tissues
Associated products
TUB Positive Extraction Control
Product codePEC-TUB
Format550 μL (freeze-dried)
Description

Freeze-dried preparation of inactivated Mycobacterium bovis BCG-Copenhagen vaccine strain (ATCC-27290) diluted in a negative lymph node supernatant.
To be prepared and extracted in the same way as samples, to validate the efficiency of the nucleic acid extraction and qPCR amplification processes and to monitor variations in analytical sensitivity.

ID Gene® Spin Universal Extraction Kit
Product codeSPIN50 / SPIN250
Format50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens

ID Gene® Mag Fast Extraction Kit
Product codeMAGFAST384
Format384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene® Mag Universal Extraction Kit
Product codeMAG192 / MAG384
Format192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.