ID Gene™ MG/MS Triplex

Real-time PCR for the qualitative detection of Mycoplasma synoviae and Mycoplasma gallisepticum in tracheal swabs and oropharyngeal swabs.

The kit simultaneously amplifies a target sequence in Mycoplasma synoviae (MS) or Mycoplasma gallisepticum (MG) bacterial genome and an endogenous control.

  • Excellent sensitivity: PCR detection limit for MG and MS: 12.5 copies / PCR
  • The most reliable MG/MS qPCR thanks to a rigorous system of controls: confirm sample presence, validate both the extraction and amplification steps and monitor variations of results over time

Other features:

  • Results in 70 minutes: extraction in 20 minutes, using the IDvet MAGFAST kit, and amplification in 50 minutes, with the IDMGMS qPCR
  • Compatible with most extraction systems and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests: test other pathogens (DNA or RNA) on the same plate
  • Ready-to-use reagents: the amplification reaction mix contains all the primers, probes and master mixrequired to run the qPCR. No pre-mixing required.
Specifications
Product code IDMGMS-50 IDMGMS-100
Reactions 50 100
Method Real time RT PCR - Triplex - Qualitative
Species Avian
Sample types Tracheal or oropharyngeal swabs
Associated extraction kits
Direct lysis buffer
Product code DLB-500
Format 500 samples
Description

Single-tube system for rapid preparation of DNA/RNA from various matrices.
ID Gene™ Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene™ Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.