Real-time RT-PCR for the qualitative detection of all serotypes of the BVD / BD viruses in ruminant blood, serum, milk, organs and ear notch samples (individual samples and pools).
This qualitative triplex test simultaneously amplifies target RNA common to all BVDV/BDV serotypes, an endogenous internal control, and a non-target exogenous positive control.
It is the only commercial BVD PCR which includes:
- positive target and non-target controls* to be extracted along with field samples to evaluate extraction efficiency and confirm the absence of inhibitors in each well;
- a target positive control calibrated at between 10 and 100 times the MDL (method detection limit) in order to monitor variations in analytical sensitivity.
Other features:
- Detects all BVD/BD serotypes
- Rapid 1 hour amplification protocol
- May also be used with direct lysis for ear notch samples (no extraction step required)
- Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
- Uses the same extraction and amplification protocols common to all ID Gene® PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
- High performance:
- DLPCR< 10 copies / PCR
- DLMethod 10DICT50/mL
- Specificity of 100%.
- Validated by the French national reference laboratory and according to the French standard NFU47-600-2.
- Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.
* Target Positive Control (TPC) included in the kit: naturally-infected, freeze-dried field sample (a freeze-dried blood or ear notch sample).
* Non-Target exogenous Positive Control (NTPC) included in the kit: non-pathogenic inactivated viral strain.
| Specifications | ||
|---|---|---|
| Product code | IDBVD-50 | IDBVD-100 |
| Reactions | 50 | 100 |
| Method | Real time RT-PCR - Triplex - Qualitative |
| Species | Ruminant |
| Sample types | Individual or pools |
| Specimens | Blood, serum, ear notch samples, organs and milk |
| Associated products |
|---|
| Direct lysis buffer for ear notches | |
| Product code | DLB-500 |
| Format | 500 preps |
| Description | Single-tube system for the rapid extraction of DNA/RNA from ear notch samples. The DLB is easy-to-use, without any liquid-handling or tube-opening steps. |
| ID Gene® Spin Universal Extraction Kit | |
| Product code | SPIN50 / SPIN250 |
| Format | 50 preps / 250 preps |
| Description | Extraction system using silica columns for all matrices and all veterinary pathogens |
| ID Gene® Mag Fast Extraction Kit | |
| Product code | MAGFAST384 |
| Format | 384 preps |
| Description | Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).
⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min! ⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification) ⇒ Compatible with the following automated extraction systems:
|
| ID Gene® Mag Universal Extraction Kit | |
| Product code | MAG192 / MAG384 |
| Format | 192 preps / 384 preps |
| Description | Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA).
⇒ Results in 40 min
⇒ Compatible with the following automated extraction systems:
⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol. |