ID Gene™ BVD/BD Triplex

Real-time RT-PCR for the qualitative detection of all serotypes of the BVD / BD viruses in ruminant blood, serum, milk, organs and ear notch samples (individual samples and pools).

This qualitative triplex test simultaneously amplifies target RNA common to all BVDV/BDV serotypes, an endogenous internal control, and a non-target exogenous positive control.

 

It is the only commercial BVD PCR which includes:

  • positive target and non-target controls* to be extracted along with field samples to evaluate extraction efficiency and confirm the absence of inhibitors in each well;
  • a target positive control calibrated at between 10 and 100 times the MDL (method detection limit) in order to monitor variations in analytical sensitivity.

Other features:

  • Detects all BVD/BD serotypes
  • Rapid 1 hour amplification protocol
  • May also be used with direct lysis for ear notch samples (no extraction step required)
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • High performance:
    • DLPCR< 10 copies / PCR
    • DLMethod 10DICT50/mL
    • Specificity of 100%.
  • Validated by the French national reference laboratory and according to the French standard NFU47-600-2.
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.

* Target Positive Control (TPC) included in the kit: naturally-infected, freeze-dried field sample (a freeze-dried blood or ear notch sample).

* Non-Target exogenous Positive Control (NTPC) included in the kit: non-pathogenic inactivated viral strain.

Specifications
Product code IDBVD-50 IDBVD-100
Reactions 50 100
Method Real time RT-PCR - Triplex - Qualitative
Species Ruminant
Sample types Individual or pools
Specimens Blood, serum, ear notch samples, organs and milk
Associated extraction kits
Direct lysis buffer for ear notches
Product code DLB-500
Format 500 preps
Description

Single-tube system for the rapid extraction of DNA/RNA from ear notch samples.

The DLB is easy-to-use, without any liquid-handling or tube-opening steps.

ID Gene™ Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens

ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene™ Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.