ID Gene™ BVD/BD Triplex

Real-time RT-PCR for the qualitative detection of all serotypes of the BVD / BD viruses in ruminant blood, serum, milk, organs and ear notch samples (individual samples and pools of up to 100).

This qualitative triplex test simultaneously amplifies target RNA common to all BVDV/BDV serotypes, an endogenous internal control, and a non-target exogenous positive control.

 

It is the only commercial BVD PCR which includes:

  • positive target and non-target controls* to be extracted along with field samples to evaluate extraction efficiency and confirm the absence of inhibitors in each well;
  • a target positive control calibrated at between 10 and 100 times the MDL (method detection limit) in order to monitor variations in analytical sensitivity.

Other features:

  • Detects all BVD/BD serotypes
  • Rapid 1 hour amplification protocol
  • May also be used with direct lysis for ear notch samples (no extraction step required)
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • High performance:
    • DLPCR< 10 copies / PCR
    • DLMethod 10DICT50/mL
    • Specificity of 100%.
  • Validated by the French national reference laboratory and according to the French standard NFU47-600-2.
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.

* Target Positive Control (TPC) included in the kit: naturally-infected, freeze-dried field sample (a freeze-dried blood or ear notch sample).

* Non-Target exogenous Positive Control (NTPC) included in the kit: negative blood sample spiked with cell culture lysat of a related pestivirus (freeze-dried).

Specifications
Product code IDBVD-50 IDBVD-100
Reactions 50 100
Method Real time RT-PCR - Triplex - Qualitative
Species Ruminant
Sample types Individual or pools of up to 100
Specimens Blood, serum, ear notch samples, organs and milk
Associated extraction kits
ID Gene™ Easy preparation for Ear Notch Samples
Product code EZNOTCH
Format 500 preps
Description

Direct lysis extraction kit for ear notch samples for use prior to testing for BVDV by qPCR (individual samples or pools of up to 100).

 

⇒ Use in combination with IDBVD: ID Gene™ BVD/BD Triplex PCR kit.

 

Benefits:

  • Rapid and easy-to-use as no extraction step is required
  • The target positive control (TPC-EN-BVD) and the non-target positive control (NTPC-EN-BVD) are included in the kit. These controls are to be extracted in the same manner as the samples to evaluate extraction efficiency and to detect the presence of PCR inhibitors.
ID Gene™ Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens

ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

 

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 90 minutes (extraction and amplification)

 

⇒ This kit is compatible with the following automated extraction systems:

  • MagMax™ Express-96 Magnetic Particles Processor (Thermo Scientific™)
  • KingFisher™ 96 (Thermo Scientific™)
  • KingFisher™ Flex (Thermo Scientific™)
  • KingFisher™ mL (Thermo Scientific™)
ID Gene™ Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

⇒ This kit is compatible with the following automated extraction systems:

  • MagMax™ Express-96 Magnetic Particles Processor (Thermo Scientific™)
  • KingFisher™ 96 (Thermo Scientific™)
  • KingFisher™ Flex (Thermo Scientific™)
  • KingFisher™ mL (Thermo Scientific™)

⇒ May be used with most commercial amplification kits (generally validated with this type of extraction protocol)