ID Gene® Brucella spp triplex

ID Gene® Brucella spp Triplex (IDBRU) is a qPCR kit that amplifies a target sequence in the Brucella spp genome.

This kit is a qualitative Triplex test. It simultaneously amplifies target DNA, as well as endogenous and exogenous internal controls. This kit may be used to test ruminant swabs (placental, vaginal, and cervical), milks, organs and tissues.

 

  • Detects all Brucella species
  • For milk testing, twice as sensitive as the gold standard method (culture)
  • Offers the most rigorous system of controls
  • Compatible with most extraction systems and all thermocyclers
Specifications
Product codeIDBRU-50IDBRU-100
Reactions50100
MethodReal time qPCR - Triplex - Qualitative
SpeciesRuminants
Sample typesSwabs (placental, vaginal, and cervical), milks, organs and tissues
Associated products
BRU Positive Extraction Control
Product codePEC-BRU
Format550 μL (freeze-dried)
Description

Freeze-dried control consisting of inactivated Brucella ovis strain, diluted into a ruminant endo-cervical swab supernatant matrix. This control is to be prepared and extracted in the same way as the samples to validate the efficiency of the nucleic acids extraction and qPCR amplification processes. It may be used to monitor variations in analytical sensitivity.

Lysis buffer
Product codeLMAP-50 / LMAP-1000
Format50 ml / 1000 ml
Description

Lysis buffer

ID Gene® Spin Universal Extraction Kit
Product codeSPIN50 / SPIN250
Format50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens

ID Gene® Mag Fast Extraction Kit
Product codeMAGFAST384
Format384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.