Productyy listReal-time RT-PCR for the qualitative detection of all serotypes of the Bluetongue virus in ruminant blood samples
This qualitative duplex test simultaneously amplifies a target sequence in the BTV genome, and an endogenous internal control.
The only commercial BTV PCR which includes a target positive control*:
- to be extracted along with field samples in order to evaluate extraction efficiency
- calibrated at between 10 and 100 times the method detection limit in order to monitor variations in analytical sensitivity
Other features:
- Detects all BTV serotypes
- Rapid (1 hour) or standard (2 hour) amplification protocols
- Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
- Uses the same extraction and amplification protocols common to all ID Gene® PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
- High performance: DLPCR< 10 copies / PCR and specificity of 100%.
- Validated by the French national reference laboratory and according to the French standard NFU47-600-2.
- Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.
* Target positive control included in the kit: inactivated BTV, diluted in a virus-negative whole blood matrix, freeze-dried and calibrated at between 10 and 100 times the detection limit of the method (MDL).
| Specifications | ||
|---|---|---|
| Product code | IDBTV-50 | IDBTV-100 |
| Reactions | 50 | 100 |
| Method | Real time RT PCR - Duplex - Qualitative |
| Species | Ruminants |
| Sample types | Blood |
| Associated extraction kits |
|---|
| ID Gene® Spin Universal Extraction Kit | |
| Product code | SPIN50 / SPIN250 |
| Format | 50 preps / 250 preps |
| Description | Extraction system using silica columns for all matrices and all veterinary pathogens |
| ID Gene® Mag Fast Extraction Kit | |
| Product code | MAGFAST384 |
| Format | 384 preps |
| Description | Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).
⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min! ⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification) ⇒ Compatible with the following automated extraction systems:
|
| ID Gene® Mag Universal Extraction Kit | |
| Product code | MAG192 / MAG384 |
| Format | 192 preps / 384 preps |
| Description | Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA).
⇒ Results in 40 min
⇒ Compatible with the following automated extraction systems:
⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol. |