ID Gene™ BlueTongue Duplex

Real-time RT-PCR for the qualitative detection of all serotypes of the Bluetongue virus in ruminant blood samples

 This qualitative duplex test simultaneously amplifies a target sequence in the BTV genome, and an endogenous internal control.

 

The only commercial BTV PCR which includes a target positive control*:

  • to be extracted along with field samples in order to evaluate extraction efficiency
  • calibrated at between 10 and 100 times the method detection limit in order to monitor variations in analytical sensitivity

Other features:

  • Detects all BTV serotypes
  • Rapid (1 hour) or standard (2 hour) amplification protocols
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • High performance: DLPCR< 10 copies / PCR and specificity of 100%.
  • Validated by the French national reference laboratory and according to the French standard NFU47-600-2.
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.

* Target positive control included in the kit: inactivated BTV, diluted in a virus-negative whole blood matrix, freeze-dried and calibrated at between 10 and 100 times the detection limit of the method (MDL).  

 

Specifications
Product code IDBTV-50 IDBTV-100
Reactions 50 100
Method Real time RT PCR - Duplex - Qualitative
Species Ruminants
Sample types Blood
Associated extraction kits
ID Gene™ Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene™ Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.