ID Gene™ African Swine Fever Duplex

ID Gene™ African Swine Fever Duplex (IDASF) is a real-time PCR kit that amplifies a target sequence in African Swine Fever (ASFV) viral genome.

It is a qualitative duplex test, which simultaneously amplifies target DNA as well as an endogenous internal control.

 

  • Validated by the EURL (CISA-INIA, Spain)
  • Includes positive controls to evaluate the efficiency of the extraction and amplification steps as well as an endogenous internal control
  • Validated to test pools of up to 20 samples, thanks to high kit sensitivity
  • Detects all ASF genotypes
  • Extensively used in ASF control programs around the world
  • Compatible with most extraction systems and all thermocyclers
  • Freeze-dried format available: ambient temperature shipping and 24-month shelf-life
Specifications
Product codeIDASF-50IDASF-100
Reactions50100
MethodqPCR - Duplex - Qualitative
SpeciesSwine, wild pig or warthog
Sample typesSerum, whole blood, oropharyngeal fluid or organ. Either individual samples or pools of generally up to 20 may be tested.
Associated products
ASF Positive Extraction Control
Product codePEC-ASF
Format550 μL (freeze-dried)
Description

For use with the liquid format of the ID Gene™ African Swine Fever Duplex kit.

Freeze-dried preparation of an inactivated ASFV strain diluted into a negative whole blood matrix. To be prepared and extracted in the same way as samples, to validate the efficiency of the nucleic acid extraction and qPCR amplification processes and to monitor variations in analytical sensitivity. Refer to the package leaflet for reconstitution and storage.

ID Gene® Spin Universal Extraction Kit
Product codeSPIN50 / SPIN250
Format50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens

ID Gene® Mag Fast Extraction Kit
Product codeMAGFAST384
Format384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene® Mag Universal Extraction Kit
Product codeMAG192 / MAG384
Format192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™96,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.