ID Gene™ African Swine Fever Duplex

Real-time PCR for the qualitative detection of African Swine Fever virus in whole blood or tissue homogenates (spleen, lymph nodes, tonsil and kidney)

This qualitative duplex test simultaneously amplifies a target sequence in the ASF genome, and an endogenous internal control.

This kit can be used to test blood or tissue homogenates (spleen, lymph nodes, tonsil or kidney).

 

Features:

  • Detects all ASF genotypes
  • Rapid 1 hour amplification protocol
  • Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
  • Uses the same extraction and amplification protocols common to all ID Gene™ PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
  • High performance: DLPCR< 2.5 copies / PCR and specificity of 100%.
  • Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.
Specifications
Product code IDASF-50 IDASF-100
Reactions 50 100
Method Real time RT PCR - Duplex - Qualitative
Species Swine
Sample types Blood or tissue homogenates (spleen, lymph nodes, tonsil or kidney)
Associated extraction kits
ID Gene™ Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

 

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.
ID Gene™ Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

 

⇒ Compatible with the following automated extraction systems:

  • IDvet’s own extraction robot: the IDEAL™,
  • the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific,
  • the BioSprint from Qiagen.

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.