ID Gene® African Swine Fever Duplex

Real-time PCR for the qualitative detection of African Swine Fever virus in whole blood or tissue homogenates (spleen, lymph nodes, tonsil and kidney)

This qualitative duplex test simultaneously amplifies a target sequence in the ASF genome, and an endogenous internal control.

This kit can be used to test blood or tissue homogenates (spleen, lymph nodes, tonsil or kidney).

Features:

Detects all ASF genotypes
Rapid 1 hour amplification protocol
Compatible with most extraction systems (magnetic beads, silica columns) and with all thermocyclers
Uses the same extraction and amplification protocols common to all ID Gene^® PCR tests so that other pathogens (DNA or RNA) may be tested on the same plate
High performance: DL_PCR< 2.5 copies / PCR and specificity of 100%.
Ready-to-use reagents mean that the amplification reaction mix contains all the primers, probes and master mix required to run the qPCR.

Specifications
Product code	IDASF-50	IDASF-100
Reactions	50	100

Method	qPCR - Duplex - Qualitative
Species	Swine
Sample types	Blood or tissue homogenates (spleen, lymph nodes, tonsil or kidney)

Associated extraction kits

ID Gene^® Spin Universal Extraction Kit
Product code	SPIN50 / SPIN250
Format	50 preps / 250 preps
Description	Extraction system using silica columns for all matrices and all veterinary pathogens

ID Gene^® Mag Fast Extraction Kit
Product code	MAGFAST384
Format	384 preps
Description	Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA). ⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min! ⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification) ⇒ Compatible with the following automated extraction systems: IDvet’s own extraction robot: the IDEAL™96, the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific, the BioSprint from Qiagen.

ID Gene^® Mag Universal Extraction Kit
Product code	MAG192 / MAG384
Format	192 preps / 384 preps
Description	Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). ⇒ Results in 40 min ⇒ Compatible with the following automated extraction systems: IDvet’s own extraction robot: the IDEAL™96, the King Fisher™ Flex and mL machines, as well as the Mag MAX™ from ThermoFisher Scientific, the BioSprint from Qiagen. ⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.