Product listID Gene™ African Swine Fever Duplex (IDASF) is a real-time PCR kit that amplifies a target sequence in African Swine Fever (ASFV) viral genome.
This duplex qPCR amplifies a target sequence in the African Swine Fever (ASFV) viral genome, as well as an endogenous internal control, for the highly sensitive and specific detection of all genotypes of ASFV DNA.
- Proven performance: Validated by the EURL (CISA-INIA, Spain) and extensively used in ASF control programs around the world
- Reliable: includes an endogenous internal control to confirm correct sample handling, sample presence and lysis. A calibrated positive extraction control (PEC-ASF) may be used to monitor variations in analytical sensitivity (sold separately).
- Practical: ready-to-use reagents facilitate testing, and proven stability allows for cost-effective shipping worldwide, without temperature control (no dry ice required).
- Flexible: may be used on multiple sample types (serum, whole blood, swabs, organ and tissue) from swine and wild boar, and for individual samples or pools of up to 20, thanks to high sensitivity
| Specifications | ||
|---|---|---|
| Product code | IDASF-50 | IDASF-100 |
| Reactions | 50 | 100 |
| Method | qPCR - Duplex - Qualitative |
| Species | Swine, wild pig or warthog |
| Sample types | Serum, whole blood, oropharyngeal fluid or organ. Either individual samples or pools of generally up to 20 may be tested. |
| Associated products |
|---|
| ASF Positive Extraction Control | |
| Product code | PEC-ASF |
| Format | 550 μL (freeze-dried) |
| Description | For use with the liquid format of the ID Gene™ African Swine Fever Duplex and Triplex kits. Freeze-dried preparation of an inactivated ASFV strain diluted into a negative whole blood matrix. To be prepared and extracted in the same way as samples, to validate the efficiency of the nucleic acid extraction and qPCR amplification processes and to monitor variations in analytical sensitivity. Refer to the package leaflet for reconstitution and storage. |
| ID Gene® Spin Universal Extraction Kit | |
| Product code | SPIN50 / SPIN250 |
| Format | 50 preps / 250 preps |
| Description | Extraction system using silica columns for all matrices and all veterinary pathogens |
| ID Gene™ Mag Fast Extraction Kit | |
| Product code | MAGFAST384 |
| Format | 384 preps |
| Description | Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA). ⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min! ⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification) ⇒ Compatible with most open extraction robots for magnetic beads |
| ID Gene® Mag Universal Extraction Kit | |
| Product code | MAG384 |
| Format | 384 preps |
| Description | Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA).
⇒ Results in 40 min ⇒ Compatible with most open extraction robots for magnetic beads ⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol. |