ID Gene™ PRRSV Triplex

ID Gene™ PRRSV Triplex is a RT-qPCR kit that amplifies two target sequences from EU and US genotypes of the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) genome.

This kit is a qualitative Triplex test. It simultaneously amplifies target RNAs as well as an exogenous internal control.
It may be used to test whole blood, serum, organ, tissue, bronchial swab, oropharyngeal fluids or cell culture supernatant.

  • Detects and differentiates both the US and EU genotypes of PRRSV
  • Detects Highly Pathogenic variants of the US genotype (HP-PRRSV)
  • Includes ready-to-use reagents, positive controls to evaluate the efficiency of the extraction and amplification steps, and an exogenous internal control
  • Shows high specificity and sensitivity on all matrices
  • Offers the fastest protocol on the market (in association with the MAGFAST extraction system)
  • Suitable for use with most extraction systems and all thermocyclers
Specifications
Product code IDPRRSV-50 IDPRRSV-100
Reactions 50 100
Method qPCR - Triplex - Qualitative
Species Swine
Sample types Whole blood, serum, organ, tissue, bronchial swab, oropharyngeal fluid, from swine or cell culture supernatant
Associated products
PRRSV Positive Extraction Control
Product code PEC-PRRSV
Format 550 μL (freeze-dried)
Description

Freeze dried preparation of inactivated PRRSV vaccinal strains (EU and US genotypes) diluted into a negative swine serum. To be prepared and extracted in the same way as samples, to validate the efficiency of the nucleic acid extraction and the qPCR amplification processes and to monitor variations in analytical sensitivity. Refer to the package leaflet for reconstitution and storage.
ID Gene® Spin Universal Extraction Kit
Product code SPIN50 / SPIN250
Format 50 preps / 250 preps
Description

Extraction system using silica columns for all matrices and all veterinary pathogens
ID Gene™ Mag Fast Extraction Kit
Product code MAGFAST384
Format 384 preps
Description

Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).

⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!

⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification)

⇒ Compatible with most open extraction robots for magnetic beads
ID Gene® Mag Universal Extraction Kit
Product code MAG192 / MAG384
Format 192 preps / 384 preps
Description

Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA). 

 

⇒ Results in 40 min

⇒ Compatible with most open extraction robots for magnetic beads

⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol.