Product listID Gene™ PRRSV Triplex is a RT-qPCR kit that amplifies two target sequences from EU and US genotypes of the Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) genome.
This kit is a qualitative Triplex test. It simultaneously amplifies target RNAs as well as an exogenous internal control.
It may be used to test whole blood, serum, organ, tissue, bronchial swab, oropharyngeal fluids or cell culture supernatant.
- Detects and differentiates both the US and EU genotypes of PRRSV
- Detects Highly Pathogenic variants of the US genotype (HP-PRRSV)
- Includes ready-to-use reagents, positive controls to evaluate the efficiency of the extraction and amplification steps, and an exogenous internal control
- Shows high specificity and sensitivity on all matrices
- Offers the fastest protocol on the market (in association with the MAGFAST extraction system)
- Suitable for use with most extraction systems and all thermocyclers
| Specifications | ||
|---|---|---|
| Product code | IDPRRSV-50 | IDPRRSV-100 |
| Reactions | 50 | 100 |
| Method | qPCR - Triplex - Qualitative |
| Species | Swine |
| Sample types | Whole blood, serum, organ, tissue, bronchial swab, oropharyngeal fluid, from swine or cell culture supernatant |
| Associated products |
|---|
| PRRSV Positive Extraction Control | |
| Product code | PEC-PRRSV |
| Format | 550 μL (freeze-dried) |
| Description | Freeze dried preparation of inactivated PRRSV vaccinal strains (EU and US genotypes) diluted into a negative swine serum. To be prepared and extracted in the same way as samples, to validate the efficiency of the nucleic acid extraction and the qPCR amplification processes and to monitor variations in analytical sensitivity. Refer to the package leaflet for reconstitution and storage. |
| ID Gene® Spin Universal Extraction Kit | |
| Product code | SPIN50 / SPIN250 |
| Format | 50 preps / 250 preps |
| Description | Extraction system using silica columns for all matrices and all veterinary pathogens |
| ID Gene® Mag Fast Extraction Kit | |
| Product code | MAGFAST384 |
| Format | 384 preps |
| Description | Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).
⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min! ⇒ Use in combination with the ID Gene® range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification) ⇒ Compatible with the following automated extraction systems:
|
| ID Gene® Mag Universal Extraction Kit | |
| Product code | MAG192 / MAG384 |
| Format | 192 preps / 384 preps |
| Description | Magnetic bead nucleic acid extraction system for use for use with any matrix or pathogen (DNA or RNA).
⇒ Results in 40 min
⇒ Compatible with the following automated extraction systems:
⇒ May be used with most commercial amplification kits which are validated with this type of extraction protocol. |