The washing system plays an important role in the quality of the final results:

• Fill the wells completely at each wash (300µl).
• Tap hard after the last wash to remove all fluid.
• Avoid drying of wells between washings. 
• Do not scrape the bottom of the wells during the washing steps, which could remove the antigen/antibody complexes.
• If using a manual wash method, overflowing of wells is not a problem. However, after the first wash it is important to empty the wells quickly to avoid contamination between wells.
• The cleanliness of the wash system may affect the quality of the results. Bacterial contamination of a tube or pump may arise if the system is never washed. We suggest that you pay particular attention to the cleanliness of the washing apparatus, regularly decontaminating the washer with a suitable detergent.