Frequently Asked Questions (FAQ)

STORAGE / SHELF LIFE / BATCHES

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

APPLICATIONS (species, DIVA, sample types)

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

TESTING PROCEDURE

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

SERUM TESTING

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

MILK TESTING

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

WASHING

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

INTERNATIONAL SERUM STANDARDS / FREEZE-DRIED POSITIVE STANDARDS

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian

TROUBLESHOOTING

What is the shelf-life of the freeze-dried serum standards (product codes beginning with “MRI”)?

We do not indicate an exact expiry date because these sera are very stable over time thanks to their freeze-dried form.

You may consider that they can be kept for up to 10 years from the production date (indicated in the upper right-hand corner of the product sheet). Store freeze-dried at +2° – 26°C.

For information regarding storage in the reconstituted (liquid) form, please refer to the product sheet.

In general, liquid sera should be stored as follows:

  • Store at -20°C in aliquots after reconstitution.
  • Never freeze/thaw more than 3 times.
  • If reconstituted, store at +4°C for up to 2 days.

Once reconstituted, the sera should be aliquoted and stored in air-tight vials (preferably with a twist cap, such as a cryotube). Otherwise, auto-lyophilisation may occur within 6-12 months at -20°C.

May the ID Screen® ELISAs be used with robots?

Yes, our kits have been used successfully with robots in many countries.

Is it possible to inter-change reagents from different batches or kits?

Yes.  Non-biological components (wash, buffers, stop, substrate) may be interchanged between products and between batches.  As much as possible, however, use the reagents delivered with the kit.

Do I need to include a reference blank on my plate?

No. A blank was included in the past in order to account for spontaneous TMB substrate colour change, or because the microplate plastic was not completely transparent.

Commercial kits no longer require the use of such a blank, as both the quality of plastic and TMB have improved over the years.

Is it possible to test pooled milk or serum samples?

Some kits allow for the testing of pooled milk or serum.  Please refer to the kit insert of the product in question.

Is it possible to differentiate between vaccinated and infected animals with ID Screen® ELISAs?

Yes, some IDvet tests allow for DIVA applications (Differentiation Infected and Vaccincated animals).

The IBRgE and Aujeszky gE tests detect naturally-infected animals in a vaccinated population, provided that animals are vaccinated with gE-deleted vaccines.

For avian influenza, if vaccination is performed using a homologous hemagglutinin to the highly pathogenic field strain (H5), but a heterologous neuraminidase subtype, serologic surveillance can be performed by testing for antibodies against the homologous N subtype (N1) as evidence of natural infection.

I am using an ID Screen® indirect multi-species test. Which species may I test?

ID Screen® indirect multi-species tests include a multi-species conjugate which recognizes mammalian IgG antibodies.
However, validation data does not exist for all species for all tests. When testing a species for which IDvet does not have validation data, check that the negative sample baseline remains low (indirect test), or that the distribution of S/N% results is not considerably altered with respect to the negative control value.

How should I store opened microplates if I do not use all the wells at once?

Opened plates are sensitive to storage conditions:

  • It is essential to immediately close the aluminium envelope after opening. 
  • Do not throw away the desiccant: the plate should be sealed with the desiccant inside the envelope. This should be carried out rapidly, as the desiccant will quickly absorb humidity and become inactivated after 10-30 minutes.
  • The end of the aluminium envelope should be cut with scissors and closed in an air-tight manner along the entire length of the envelope, with scotch tape, for example. (Simply folding-over the edges and/or stapling is insufficient to ensure sealing.). Please refer to the photos on the document Plate storage.pdf
  • Store at 5°C ± 3°C.

What is the shelf life/expiry date of the ID Screen® ELISA kits?

The majority of ID Screen® kits have a shelf-life of 24 months from the production date.

How do I prepare the 10x conjugate for use?

Please refer to the document 1x Conjugate preparation conditions
also available in French version: Conditions de préparation du conjugué 1x
and Russian version: 1x Conjugate preparation conditions in Russian