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The article presents data which demonstrates the advantages offered by the IDvet mycoplasma ELISAs.
The ELISA range offers a number of advantages:
- High exclusivity, with no cross-reactions between different mycoplasmas;
- Early and long-term antibody detection and high correlation with the Rapid Slide Agglutination (RSA) test;
- The best combination of sensitivity and specificity among commercial ELISAs.
No cross-reactions between mycoplasmas thanks to the use of recombinant proteins
28-week old layers from a flock in Turkey were tested in parallel with commercial Kit A and the ID Screen® MG test.
While 11/21 (52%) samples were positive on Kit A, all animals were negative on the ID Screen® MG ELISA (Figure 1).
When tested with the ID Screen MS® ELISA, however, this panel gave positive results for MS with very high titers (see Figure 2).
Samples were also tested by RSA after de-complementation (56°C – 30 min): samples were positive with the MS antigen, and negative with the MG antigen (Figure 3).
Figure 3 : an example of results obtained for 28-week layer samples with RSA MS (BIOVAC, ref AS6) and MG (BIOVAC, ref AS9) tests
This study illustrates how cross-reactions may be obtained with competitors’ ELISAs. IDvet ELISAs show high exclusivity thanks to the use of recombinant protein antigens.
The test detects IgG and IgM antibodies, allowing for early and long-term antibody detection and high correlation with RSA
In an experimental study performed by MSD, SPF layers were vaccinated with the Nobilis® MS live vaccine and tested with different MS ELISAs at 2, 3, 4 and 8 weeks post-vaccination (wpv).
Depending on the kit used, the kinetics of vaccine seroconversion varied:
Data kindly provided by Rick Koopman, MSD
Commercial kit 1 detected only 10% of animals 2 wpv, with positivity reaching 70% at 8 wpv, indicating detection of IgG antibodies only.
Commercial kit 2 detected 90% of animals as of 2 wpv, with the rate decreasing to only 20% at 8 weeks post-vaccination, indicating detection of IgM antibodies only.
The ID Screen® MS kit detected more than 85% of animals as of 2 wpv, and this rate remained stable over time. The positivity rate was 70% at 8 wpv, indicating detection of both IgG and IgM antibodies.
The IDvet mycoplasma ELISAs offer the best combination of sensitivity and specificity among commercial ELISAs
Early detection, including the 6/85 vaccine, of the GD Deventer Proficiency Scheme panel
In a GD Deventer proficiency testing scheme for mycoplasma, the IDvet MS ELISA detected seroconversion as of 7 days post-infection, whereas Kit A gave negative results.
The IDvet MG test is the only test which detected the 6/85 strain as of 4 wpv in the GD Deventer Proficiency Testing Scheme; the two other ELISAs gave negative results (Table 2).
Efficient and early detection of the 6/85 vaccine
Chicken flocks were vaccinated only once at the age of 5 weeks with either the ts-11 vaccine by eye-drop or the 6/85 vaccine by spray.
The 6/85 and ts-11 strains were kindly provided by the University of Melbourne, Australia.
Samples were tested in parallel at 4 and 30 or 36 wpv using the ID Screen® ELISA, another commercial ELISA (Kit D) and a commercial MG RSA antigen (Table 3).
The ID Screen® ELISA detected animals vaccinated with the 6/85 strain even at 4 weeks post-vaccination, and showed good agreement with the RSA test.
In conclusion, IDvet offers a complete range of ELISAs to monitor and control Mycoplasma in poultry flocks, offering reliable and accurate results.
With highly pathogenic H5N8 rapidly spreading throughout Europe, IDvet offers a range of highly specific and sensitive Influenza ELISAs to efficiently monitor spread of the disease.
The ID Screen® Influenza H5 competitive ELISA has been validated by several European reference laboratories to detect new circulating clades, including H5N8. The test may be used to screen for H5 antibodies and/or monitor rHVT-H5 vaccine uptake. It may also be used to confirm positive results obtained with the nucleoprotein-based ELISAs.
If screening for disease in chickens and turkeys only, use the ID Screen® Influenza A Nucleoprotein Indirect ELISA which offers the highest sensitivity of any Influenza antibody test on the market. As it specifically detects NP antibodies, the test can be used to detect natural infection in animals vaccinated with rHVT-H5 vaccines alone. The test may also be used to monitor killed vaccines and is ideally suited to monitoring disease in SPF animals thanks to its high sensitivity.
If testing both domestic and wild avian species in regions without vaccination, choose the ID Screen® Influenza A Competitive ELISA, which detects anti-nucleoprotein (NP) antibodies in multiple species.
Many countries, including France and Germany, are using IDvet products to monitor the current outbreaks, screening with the nucleoprotein test and confirming (or screening) with the H5 ELISA.
As an expert in the development of Influenza serological tools, IDvet also offers ELISAs which specifically detect H7, H9, N1, N2 and N9 antibodies.