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Swine Vesicular Disease

 
ID Screen® Swine Vesicular Disease Competition ELISA kit

Swine vesicular disease (SVD) is a contagious viral disease of pigs characterized by fever and vesicles in the mouth and on the snout, feet and teats. The illness may be subclinical, mild or severe, but rarely fatal. The importance of this disease stems from the fact that it cannot be clinically distinguished from foot-and-mouth disease (FMD). Outbreaks of SVD are therefore assumed to be FMD until laboratory tests prove otherwise.
Serology is used for SVD surveillance and export certification.  While virus neutralization is the prescribed test for international trade, it is time-consuming, and 2-3 days are required to obtain results. In contrast, results may be obtained by ELISA in less than 2 hours.

IDVET has developed the ID Screen Swine Vesicular Disease Competition ELISA. The method used in this kit is described in the OIE Manual for Terrestrial Animals, Chapter 2.3.1. It is based on the ELISA developed by Brocchi et al (1) and is standardized to detect the Primary Reference Serum EU-RS4.

Product Code
  • SVDC
Description
  • Competitive ELISA
  • Detects anti-SVD antibodies
  • Standardized to detect the Primary Reference Serum EU-RS4.
Applications
  • Porcine sera
Format
  • 384 reactions
  • 4 plates in strips of 8 microwells
Advantages
  • Results in 1 hour and 45 minutes
  • Ready-to-use components
References
  • (1) Brocchi E., Berlinzani A., Gamba D. & De Simone F. (1995). Development of two novel monoclonal antibody-based ELISAs for the detection of antibodies and the identification of swine isotypes against swine vesicular disease virus. J. Virol. Methods, 52, 155-67.
  • (2)OIE Manual for Terrestrial Animals, Chapter 2.3.1
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